Academic Journal
Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities
| العنوان: | Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities |
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| المؤلفون: | Perinelli M., Guerrini R., Albanese V., Marchetti N., Bellotti D., Gentili S., Tegoni M., Remelli M. |
| المساهمون: | Perinelli, M., Guerrini, R., Albanese, V., Marchetti, N., Bellotti, D., Gentili, S., Tegoni, M., Remelli, M. |
| بيانات النشر: | Elsevier Inc. |
| سنة النشر: | 2020 |
| المجموعة: | Università di Parma: CINECA IRIS |
| مصطلحات موضوعية: | Branched peptide, Copper(II), Cyclam scaffold, Metal complexe, Peptide welding technology, Solution equilibria |
| الوصف: | The two branched peptides (AAHAWG)4-PWT2 and (HAWG)4-PWT2 where synthesized by mounting linear peptides on a cyclam-based scaffold (PWT2), provided with four maleimide chains, through a thio-Michael reaction. The purpose of this study was primarily to verify if the two branched ligands had a Cu(II) coordination behavior reproducing that of the single-chain peptides, namely AAHAWG-NH2, which bears an Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) Motif, and HAWG-NH2, which presents a His residue as the N-terminal amino acid, in a wide pH range. The study of Cu(II) binding was performed by potentiometric, spectroscopic (UV–vis absorption, CD, fluorescence) and ESI-MS techniques. ATCUN-type ligands ((AAHAWG)4-PWT2 and AAHAWG-NH2) were confirmed to bind one Cu(II) per peptide fragment at both pH 7.4 and pH 9.0, with a [NH2, 2N−, NIm] coordination mode. On the other hand, the ligand HAWG-NH2 forms a [CuL2]2+ species at neutral pH, while, at pH 9, the formation of 1:2 Cu(II):ligand adducts is prevented by amidic nitrogen deprotonation and coordination, to give rise solely to 1:1 species. Conversely, Cu(II) binding to (HAWG)4-PWT2 resulted in the formation of 1:2 copper:peptide chain also at pH 9: hence, through the latter branched peptide we obtained, at alkaline pH, the stabilization of a specific Cu(II) coordination mode which results unachievable using the corresponding single-chain peptide. This behavior could be explained in terms of high local peptide concentration on the basis of the speciation of the Cu(II)/single-chain peptide systems. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000522119300008; volume:205; firstpage:110980; journal:JOURNAL OF INORGANIC BIOCHEMISTRY; http://hdl.handle.net/11381/2886221; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85077661872 |
| DOI: | 10.1016/j.jinorgbio.2019.110980 |
| الاتاحة: | http://hdl.handle.net/11381/2886221 https://doi.org/10.1016/j.jinorgbio.2019.110980 |
| رقم الانضمام: | edsbas.2692555A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jinorgbio.2019.110980 |
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