Academic Journal
Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus
| العنوان: | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
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| المؤلفون: | Asins, María J., Bullones, Amanda, Raga, Verónica, Romero-Aranda, María R., Espinosa, Jesús, Triviñi, Juan C., Bernet, Guillermo P., Traverso, Jose A., Carbonell, Emilio A., Claros-Díaz, Manuel Gonzalo, Belver, Andres |
| المصدر: | electronico |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | QTL analysis, Citrus reshni, Rootstock breeding, Root growth, Cl-Homeostasis, F30 Plant genetics and breeding, U30 Research methods, F62 Plant physiology - Growth and development, P33 Soil chemistry and physics, QTL (quantitative trait loci), Poncirus trifoliata, Yields, Plasticity |
| الوصف: | The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | The study was co-funded by grants RTA2011-00132-C2 (M.J.A.), AGL2014-56675-R (M.J.A.), AGL2017-82452-C2 (A.Be., M.J.A.), and PID2021-124599OB-I00 (A.Be.) from the Spanish Ministry of Science and Innovation/the Spanish Research Agency, by grant 51917 (M.J.A.) from the Generalitat Valenciana, as well as the European Regional Development Fund (ERDF), MCIN/AEI/10.13039/501100011033, and ERDF “A way of making Europe”.; info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/RTA2011-00132-C02-02/ES/Pre-Mejora genética en limonero; info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-56675-R/ES/Aprovechamiento de recursos fitogenéticos para la diversificación de patrones eficientes en la adquisición de hierro; info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2017- 82452-C2/ES/Transportadores de iones probablemente implicados en QTLS de gran efecto sobre la tolerancia a salinidad en términos de producción en cítricos y tomate; info:eu-repo/grantAgreement/AEI/Proyectos de Generación de Conocimiento/PID2021-124599OB-100; Asins, M. J., Bullones, A., Raga, V., Romero-Aranda, M. R., Espinosa, J., Triviño, J. C. et al. (2023). Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus. International Journal of Molecular Sciences, 24(21), 15759.; https://hdl.handle.net/20.500.11939/8737; https://www.mdpi.com/1422-0067/24/21/15759; https://www.ebi.ac.uk/ena/browser/view/PRJEB61142; https://www.ncbi.nlm.nih.gov/bioproject/1070151 |
| DOI: | 10.3390/ijms242115759 |
| الاتاحة: | https://hdl.handle.net/20.500.11939/8737 https://doi.org/10.3390/ijms242115759 https://www.mdpi.com/1422-0067/24/21/15759 https://www.ebi.ac.uk/ena/browser/view/PRJEB61142 https://www.ncbi.nlm.nih.gov/bioproject/1070151 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; openAccess |
| رقم الانضمام: | edsbas.268605B8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms242115759 |