التفاصيل البيبلوغرافية
| العنوان: |
Ergosterol peroxide: an effect-directed detecting method from Anoectochilus elwesii and evaluation of anticancer activity |
| المؤلفون: |
Yanduan Lin (11126177), Bingqin Wen (11126180), Ziwei Zhang (2029975), Jinyan Cai (590861) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Physiology, Cancer, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Ergosterol peroxide, endoperoxide bridge, DPPH, cytotoxicity, reactive oxygen species, Anoectochilus elwesii |
| الوصف: |
Ergosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecting method of HPTLC-DPPH/UHPLC/ESI-TOF-MS was developed for EP rapidly and precisely, providing an option for identifying oxidative compounds. EP exhibits high cytotoxic activity against SGC-7901 gastric cancer cells and the morphological apoptosis suggested that EP induced apoptosis and cell cycle arrest in G0/G1 phase. It could enhance the ROS level and cause a decrease in the mitochondrial membrane potential. Its antiproliferative mechanism is G0/G1 phase arrest and might be traced through ROS-mediated mitochondrial dysfunction pathways. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Ergosterol_peroxide_an_effect-directed_detecting_method_from_i_Anoectochilus_elwesii_i_and_evaluation_of_anticancer_activity/14974619 |
| DOI: |
10.6084/m9.figshare.14974619.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.14974619.v1 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.2658498D |
| قاعدة البيانات: |
BASE |