Academic Journal
The use of a complex tetra-culture alveolar model to study the biological effects induced by gold nanoparticles with different physicochemical properties
| العنوان: | The use of a complex tetra-culture alveolar model to study the biological effects induced by gold nanoparticles with different physicochemical properties |
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| المؤلفون: | Saibene, Melissa, Serchi, Tommaso, Bonfanti, Patrizia, Colombo, Anita, Nelissen, Inge, Halder, Rashi, Audinot, Jean-Nicolas, Pelaz, Beatriz, Soliman, Mahmoud G., Parak, Wolfgang J., Mantecca, Paride, Gutleb, Arno C., Cambier, Sebastien |
| المساهمون: | Saibene, M, Serchi, T, Bonfanti, P, Colombo, A, Nelissen, I, Halder, R, Audinot, J, Pelaz, B, Soliman, M, Parak, W, Mantecca, P, Gutleb, A, Cambier, S |
| بيانات النشر: | Elsevier NL |
| سنة النشر: | 2024 |
| المجموعة: | Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) |
| مصطلحات موضوعية: | Air-liquid-interface, Alveolar in vitro model, Gold nanoparticle, Transcriptomic |
| الوصف: | A substantial increase in engineered nanoparticles in consumer products has been observed, heightening human and environmental exposure. Inhalation represents the primary route of human exposure, necessitating a focus on lung toxicity studies. However, to avoid ethical concerns the use of in vitro models is an efficient alternative to in vivo models. This study utilized an in vitro human alveolar barrier model at air-liquid-interface with four cell lines, for evaluating the biological effects of different gold nanoparticles. Exposure to PEGylated gold nanospheres, nanorods, and nanostars did not significantly impact viability after 24h, yet all AuNPs induced cytotoxicity in the form of membrane integrity impairment. Gold quantification revealed cellular uptake and transport. Transcriptomic analysis identified gene expression changes, particularly related to the enhancement of immune cells. Despite limited impact, distinct effects were observed, emphasizing the influence of nanoparticles physicochemical parameters while demonstrating the model's efficacy in investigating particle biological effects. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38163529; info:eu-repo/semantics/altIdentifier/wos/WOS:001157367700001; volume:106; issue:March 2024; journal:ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY; https://hdl.handle.net/10281/464898; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85182252010 |
| DOI: | 10.1016/j.etap.2023.104353 |
| الاتاحة: | https://hdl.handle.net/10281/464898 https://doi.org/10.1016/j.etap.2023.104353 |
| رقم الانضمام: | edsbas.263D0DD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.etap.2023.104353 |
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