Academic Journal
ZART: A Novel Multiresolution Reconstruction Algorithm with Motion-blur Correction for Single Particle Analysis
| العنوان: | ZART: A Novel Multiresolution Reconstruction Algorithm with Motion-blur Correction for Single Particle Analysis |
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| المؤلفون: | Herreros, D, Kiska, J, Ramírez-Aportela, E, Filipovic, J, Carazo, J M, Sorzano, C O S |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Air Force Office of Scientific Research (US), National Institute of General Medical Sciences (US), Ramírez-Aportela, Erney, Carazo, José M., Sorzano, Carlos Óscar S |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Cryo-Electron Microscopy (CryoEM), Zernike polynomials, Zernike3D-based Algebraic Reconstruction Technique (ZART), map reconstruction, spherical harmonics |
| الوصف: | Supplementary data associated with this article can be found, in the online version, at https://doi. org/10.1016/j.jmb.2023.168088. ; One of the main purposes of CryoEM Single Particle Analysis is to reconstruct the three-dimensional structure of a macromolecule thanks to the acquisition of many particle images representing different poses of the sample. By estimating the orientation of each projected particle, it is possible to recover the underlying 3D volume by multiple 3D reconstruction methods, usually working either in Fourier or in real space. However, the reconstruction from the projected images works under the assumption that all particles in the dataset correspond to the same conformation of the macromolecule. Although this requisite holds for some macromolecules, it is not true for flexible specimens, leading to motion-induced artefacts in the reconstructed CryoEM maps. In this work, we introduce a new Algebraic Reconstruction Technique called ZART, which is able to include continuous flexibility information during the reconstruction process to improve local resolution and reduce motion blurring. The conformational changes are modelled through Zernike3D polynomials. Our implementation allows for a multiresolution description of the macromolecule adapting itself to the local resolution of the reconstructed map. In addition, ZART has also proven to be a useful algorithm in cases where flexibility is not so dominant, as it improves the overall aspect of the reconstructed maps by improving their local and global resolution. ; Funding is acknowledged from the Ministry of Science and Innovation through grants: Grant PID2019-104757RB-I00 funded by MCIN/AEI/10.1 3039/501100011033/ and “ERDF A way of making Europe”, by the “European Union; the ‘Comunidad Autonoma de Madrid’ through grant S2017/BMD- 3817; and the European Union (EU) and Horizon 2020 through grants EnLaCES (H2020-MSCA-IF- 2020, Proposal: 101024130 to JMK), HighResCells (ERC-2018-SyG, Proposal: 810057) and iNEXT-Discovery (Proposal: 871037). This ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-2836 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104757RB-I00/ES/AIM-CRYOEM: PROCESAMIENTO DE IMAGEN AVANZADO ORIENTADO AL ANALISIS DE PARTICULAS INDIVIDUALES EN MICROSCOPIA ELECTRONICA EN CONDICIONES CRIOGENICAS/; Journal of molecular biology; Publisher's version; application/pdf; https://www.sciencedirect.com/science/article/pii/S002228362300150X; Sí; Journal of Molecular Biology 2023; 435: 168088; http://hdl.handle.net/10261/364258; 2-s2.0-85152735879; https://api.elsevier.com/content/abstract/scopus_id/85152735879 |
| DOI: | 10.1016/j.jmb.2023.168088 |
| الاتاحة: | http://hdl.handle.net/10261/364258 https://doi.org/10.1016/j.jmb.2023.168088 https://api.elsevier.com/content/abstract/scopus_id/85152735879 |
| Rights: | open |
| رقم الانضمام: | edsbas.25E6049E |
| قاعدة البيانات: | BASE |
| تدمد: | 00222836 |
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| DOI: | 10.1016/j.jmb.2023.168088 |