Academic Journal
Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring
| العنوان: | Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring |
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| المؤلفون: | Shaik, Faruk Azam, Lewuillon, Clara, Guillemette, Aurélie, Ahmadian, Bahram, Brinster, Carine, Quesnel, Bruno, Collard, Dominique, Touil, Yasmine, Lemonnier, Loïc, Tarhan, Mehmet |
| المساهمون: | Université de Lille, Centre National de la Recherche Scientifique (CNRS), Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), JUNIA (JUNIA), Université catholique de Lille (UCL), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie Cellulaire - U 1003 (PHYCELL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, This work is in the framework of SMMiL-E activities. F. A. Shaik is a recipient from a Contrat de Plan Etat-Région CPER Cancer 2015-2020 fellowship. This work is supported by grants from Contrat de Plan Etat-Région CPER Cancer 2015-2020, INSERM, CNRS, Ligue contre le cancer (Septentrion), Ligue nationale contre le cancer, ARC, and the Institut de Recherche sur le Cancer de Lille (IRCL). The authors acknowledge IRCL for hosting SMMiL-E. M. C. Tarhan acknowledges Fondation ARC. We would also like to thank Nathalie Jouy (Flow Cytometry Platform, Univ Lille, Lille, France) for her technical assistance, and Jean-Claude Gerbedoen (LIMMS, Lille, France) for his assistance on fabrication. Clara Lewuillon is financed by Lille Hospital and by Hauts de France Region. |
| المصدر: | ISSN: 1473-0197. |
| بيانات النشر: | HAL CCSD Royal Society of Chemistry |
| سنة النشر: | 2022 |
| المجموعة: | Université Polytechnique Hauts-de-France: HAL |
| مصطلحات موضوعية: | [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics |
| الوصف: | International audience ; Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting cells of similar dimensions. T lymphocytes are smaller, more deformable, and more sensitive to pressure than many cells. This work aims to fill the gap of a method for pairing cells with different dimensions. The developed method uses hydrodynamic flow focusing in the z-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-03565627; https://hal.science/hal-03565627; https://hal.science/hal-03565627/document; https://hal.science/hal-03565627/file/Faruk%20Azam%20Shaik_2022_LOC_d1lc01156a.pdf; WOS: 000748631100001 |
| DOI: | 10.1039/d1lc01156a |
| الاتاحة: | https://hal.science/hal-03565627 https://hal.science/hal-03565627/document https://hal.science/hal-03565627/file/Faruk%20Azam%20Shaik_2022_LOC_d1lc01156a.pdf https://doi.org/10.1039/d1lc01156a |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.25B20FE8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/d1lc01156a |
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