التفاصيل البيبلوغرافية
| العنوان: |
Loss of ATRX and SLF2 enables telomere exchanges. |
| المؤلفون: |
William A. Scott (11707932), Erum Z. Dhanji (11707935), Boris J. A. Dyakov (11707938), Ema S. Dreseris (11707941), Jonathon S. Asa (11707944), Laura J. Grange (7848448), Mila Mirceta (11707947), Christopher E. Pearson (263866), Grant S. Stewart (11707950), Anne-Claude Gingras (154225), Eric I. Campos (11707953) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Cancer, Hematology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, identify proximal associations, helicase protein associates, repetitive dna regions, dna repair factors, deposit histone h3, atrx proximal associations, daxx histone chaperone, new associating proteins, dependent chromatin remodelling, chromatin remodelling, histone deposition, dna replication, dependent biotinylation, associating proteins, chromatin maintenance, %22">xlink ">, used proximity, study expands, ribosome biosynthesis, proteomic changes |
| الوصف: |
A: DNA sequencing and western blotting confirming the disruption of ATRX expression in CRISPR-Cas9-edited HEK293 Flp-In T-REx cells inducibly expressing RAP1-BirA*. B: Relative signal overlap between immunolabeled SLF2 and telomere fluorescence in situ hybridization (IF-FISH), in ATRX-expressing or -null HEK293 Flp-In cells. Cells were exposed to 10 μM pyridostatin (PDS, G4 stabilizer) for 60 min. At least 40 cells per condition were analyzed using a CellProfiler colocalization pipeline [ 106 ]. ATRX loss decreased SLF2 recruitment to telomeres in the absence of pyridostatin. C: Schematic of dual-colour chromosome-orientation fluorescence in situ hybridization (CO-FISH) [ 64 ] using PNA probes to label C- and G-rich telomere strands (left). Examples of normal CO-FISH signals and of a chromosome end with a telomere exchange are shown (right). D: Relative amount of telomere exchanges in ATRX, SLF2, and ATRX/SLF2 KO HEK293 Flp-In cells. At least 30 mitotic spreads per condition were counted and the percent of telomeric exchanges plotted. p-values were obtained using a 2-sided Student’s t-test with unequal variance. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Loss_of_ATRX_and_SLF2_enables_telomere_exchanges_/17017302 |
| DOI: |
10.1371/journal.pgen.1009909.g004 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009909.g004 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.254872B4 |
| قاعدة البيانات: |
BASE |