Academic Journal
Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries
| العنوان: | Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries |
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| المساهمون: | Yuko Ishikawa, Hiroko Ida-Yonemochi, Hironobu Suzuki, Kuniko Nakakura-Ohshima, Han-Sung Jung, Masaki J. Honda, Yumiko Ishii, Nobukazu Watanabe, Hayato Ohshima, Jung, Han Sung |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult Stem Cells/physiology, Animals, Bromodeoxyuridine, Cell Differentiation, Cell Proliferation, Dental Cavity Preparation, Dental Pulp/cytology, Dental Pulp/physiology, Female, Mesenchymal Stromal Cells/cytology, Pregnancy, Rats, Wistar, Regeneration, Side-Population Cells/cytology, Tooth Injuries/physiopathology, Tooth Replantation, Rats (Wistar) |
| الوصف: | Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76%) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 227~241 |
| اللغة: | unknown |
| تدمد: | 0948-6143 1432-119X |
| Relation: | HISTOCHEMISTRY AND CELL BIOLOGY; J00992; OAK-2010-01219; https://ir.ymlib.yonsei.ac.kr/handle/22282913/101619; http://link.springer.com/article/10.1007%2Fs00418-010-0727-5; T201002500; HISTOCHEMISTRY AND CELL BIOLOGY, Vol.134(3) : 227-241, 2010 |
| DOI: | 10.1007/s00418-010-0727-5 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101619 https://doi.org/10.1007/s00418-010-0727-5 http://link.springer.com/article/10.1007%2Fs00418-010-0727-5 |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ |
| رقم الانضمام: | edsbas.2463BF1A |
| قاعدة البيانات: | BASE |
| تدمد: | 09486143 1432119X |
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| DOI: | 10.1007/s00418-010-0727-5 |