التفاصيل البيبلوغرافية
| العنوان: |
Disparate effects of sclerostin deletion on alveolar bone and cellular cementum in mice |
| المؤلفون: |
Phanrungsuwan, Aonjittra, Chavez, Michael B., Eltilib, Leena A., Kolli, Tamara N., Mohamed, Fatma F., Tan, Michelle H., Salmon, Cristiane R., Nociti, Francisco H., Foster, Brian L. |
| المساهمون: |
National Institute of Dental and Craniofacial Research |
| المصدر: |
Journal of Periodontology ; ISSN 0022-3492 1943-3670 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Background Cellular cementum (CC) includes cementocytes, cells suspected to regulate CC formation or resorption as osteocytes do in bone. Sclerostin (SOST) is a secreted negative regulator of Wnt/β‐catenin signaling expressed by osteocytes and cementocytes. Osteocyte SOST expression reduces bone formation. We investigated the functional importance of SOST in CC compared with alveolar bone (AB) using a Sost knockout ( Sost −/− ) mouse model to better understand the role of cementocytes in CC. Methods Mandibles and femurs of Sost −/− and wild‐type (WT) mice were analyzed at 42 and 120 days postnatal (dpn). Maxillary first molars were bilaterally extracted at 42 dpn and both AB healing (maxillary molar sockets) and CC apposition (mandibular first molars) were examined at 21 days post‐procedure. Analyses included micro‐computed tomography, histology, and immunohistochemistry. Results Femur cortical and trabecular bone and mandibular bone volumes were similarly increased in Sost −/− versus WT mice at 42 and/or 120 dpn. In contrast to previous reports, CC was not increased by Sost −/− at either age. We conducted challenge experiments on AB and CC to explore tissue‐specific responses. Post‐extraction AB healing was improved by Sost deletion. In contrast, experimentally‐induced apposition in molars failed to stimulate increased CC formation in Sost −/− versus WT mice. Wnt pathway markers AXIN2 and DKK1, which were increased in Sost −/− versus WT AB osteocytes, were unchanged in cementocytes. Conclusions These data indicate CC is less responsive than AB to SOST deletion. Within the study limitations, these results do not support cementocytes as critical for directing increased CC formation. Plain language summary Sclerostin is a protein known to inhibit bone formation, and removing sclerostin leads to more bone formation. Cementum is the thin layer that covers the surface of the tooth's root. Previous studies suggest that inhibiting sclerostin can similarly increase the amount of cementum. We wanted to compare ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/jper.24-0025 |
| الاتاحة: |
http://dx.doi.org/10.1002/jper.24-0025 |
| Rights: |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.244B0302 |
| قاعدة البيانات: |
BASE |