التفاصيل البيبلوغرافية
| العنوان: |
First detection of “Candidatus Mycoplasma haemolamae” in alpaca (Vicugna pacos) in Italy |
| المؤلفون: |
Giulia Sala, Gabriele Ratti, Vincenzo Ferrulli, Donatella Scavone, Angelica Stranieri, Alessia Giordano, Antonio Boccardo, Davide Pravettoni, Stefania Lauzi |
| المساهمون: |
G. Sala, G. Ratti, V. Ferrulli, D. Scavone, A. Stranieri, A. Giordano, A. Boccardo, D. Pravettoni, S. Lauzi |
| سنة النشر: |
2022 |
| المجموعة: |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: |
Alpaca, Candidatus Mycoplasma haemolamae, Anaemia, Settore VET/08 - Clinica Medica Veterinaria, Settore VET/05 - Malattie Infettive degli Animali Domestici |
| الوصف: |
Objective: Candidatus Mycoplasma haemolamae is a wall-less hemotropic prokaryote that infects camelids. To the author's knowledge, there have been no published reports of Candidatus M. haemolamae infection in alpacas in Italy. This study describes a clinical case of Candidatus M. haemolamae infection in an alpaca cria from northern Italy and the prevalence in its herd. Materials and methods: A 2 month-old alpaca cria was referred to the Clinic for Ruminants of the Veterinary Teaching Hospital of the University of Milan for weakness and lethargy. At birth, the alpaca cria was immediately rejected by their dam and fed with artificial bovine colostrum (Locatim®, Boehringer Ingelheim, Germany) and pasteurized whole cow's milk. At admission, the cria presented pale mucosae, tachycardia, and moderate dehydration. The hemogasanalysis underlined severe hypoglycaemia (1.1 mmol/L) and anaemia (haematocrit 17%, haemoglobin 5.6 g/dl). The complete blood count (CBC) confirmed mild, regenerative anaemia. The blood smear revealed numerous small basophilic coccoid structures attached to the surface of erythrocytes compatible with Candidatus M. haemolamae infection. In addition, parasitology examination of the faeces revealed severe coccidian infestation. To confirm the presence of Candidatus M. haemolamae, a portion of the 16S rRNA gene was amplified using a species-specific real-time PCR on blood samples collected at day of admission and at day of discharge. Threshold cycle (Ct) number was used as the measure of bacterial load (the lower the Ct level the greater the amount of target nucleic acid is present in the sample). Subsequently, the other animals of the entire herd from which the primary case was detected were tested by real-time PCR and by blood smear examination to investigate the presence of Candidatus M. haemolamae (n=20). Results: In the blood sample collected at admission, real-time PCR revealed a high level of Candidatus M. haemolamae DNA (Ct 11.7). The cria was stabilized by administration of a 10% glucose ... |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| Relation: |
World Buiatrics Congress; https://hdl.handle.net/2434/939608 |
| الاتاحة: |
https://hdl.handle.net/2434/939608 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.243356E |
| قاعدة البيانات: |
BASE |