Academic Journal
Anti-nociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain.
| العنوان: | Anti-nociceptive effect of peripheral serotonin 5-HT2B receptor activation on neuropathic pain. |
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| المؤلفون: | Urtikova, Nataliya, Berson, Nadège, van Steenwinckel, Juliette, Doly, Stéphane, Truchetto, Jérémy, Maroteaux, Luc, Pohl, Michel, Conrath, Marie |
| المساهمون: | Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | ISSN: 0304-3959. |
| بيانات النشر: | HAL CCSD Lippincott, Williams & Wilkins |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MESH: Animals, MESH: Disease Models, Animal, MESH: Serotonin 5-HT2 Receptor Agonists, MESH: Thiophenes, MESH: Ganglia, Spinal, MESH: Indoles, MESH: Male, MESH: Neuralgia, MESH: Nociceptors, MESH: Rats, Sprague-Dawley, MESH: Receptor, Serotonin, 5-HT2B, MESH: Sciatic Nerve, MESH: Sciatic Neuropathy, MESH: Serotonin, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology |
| الوصف: | International audience ; Serotonin is critically involved in neuropathic pain. However, its role is far from being understood owing to the number of cellular targets and receptor subtypes involved. In a rat model of neuropathic pain evoked by chronic constriction injury (CCI) of the sciatic nerve, we studied the role of 5-HT(2B) receptor in dorsal root ganglia (DRG) and the sciatic nerve. We showed that 5-HT(2B) receptor activation both prevents and reduces CCI-induced allodynia. Intrathecal administration of 5-HT(2B) receptor agonist BW723C86 significantly attenuated established mechanical and cold allodynia; this effect was prevented by co-injection of RS127445, a selective 5-HT(2B) receptor antagonist. A single application of BW723C86 on the sciatic nerve concomitantly to CCI dose-dependently prevented mechanical allodynia and significantly reduced cold allodynia 17 days after CCI. This behavioral effect was accompanied with a marked decrease in macrophage infiltration into the sciatic nerve and, in the DRG, with an attenuated abnormal expression of several markers associated with local neuroinflammation and neuropathic pain. CCI resulted in a marked upregulation of 5-HT(2B) receptor expression in sciatic nerve and DRG. In the latter structure, it was biphasic, consisting of a transient early increase (23-fold), 2 days after the surgery and before the neuropathic pain emergence, followed by a steady (5-fold) increase, that remained constant until pain disappeared. In DRG and sciatic nerve, 5-HT(2B) receptors were immunolocalized on sensory neurons and infiltrating macrophages. Our data reveal a relationship between serotonin, immunocytes, and neuropathic pain development, and demonstrate a critical role of 5-HT(2B) receptors in blood-derived macrophages. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22525520; hal-01225065; https://hal.science/hal-01225065; https://hal.science/hal-01225065/document; https://hal.science/hal-01225065/file/PAIN-S-11-01032.pdf; PUBMED: 22525520 |
| DOI: | 10.1016/j.pain.2012.03.024 |
| الاتاحة: | https://hal.science/hal-01225065 https://hal.science/hal-01225065/document https://hal.science/hal-01225065/file/PAIN-S-11-01032.pdf https://doi.org/10.1016/j.pain.2012.03.024 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.23F583C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.pain.2012.03.024 |
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