Academic Journal
Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture
| العنوان: | Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture |
|---|---|
| المؤلفون: | Sevgi Karabulut Uzunçakmak, Zekai Halıcı, Songül Karakaya, Zerrin Kutlu, Yavuz Selim Sağlam, İsmail Bolat, Pelin Aydın, Ceyda Sibel Kılıç |
| المصدر: | Journal of Investigative Surgery, Vol 36, Iss 1 (2023) |
| بيانات النشر: | Taylor & Francis Group |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | oxidative stress, rat, sepsis, suberosin, lung injury, Surgery, RD1-811 |
| الوصف: | Background/aims Sepsis is one of the major problems encountered in intensive care units, causing organ damage and increasing mortality. Suberosin (SBR) is a type of coumarin with antioxidant and anti-inflammatory activities. The goal of this study is to explore the protective effects of SBR on the lungs in a rat model of sepsis. Methods Male Wistar rats were utilized in this study. A cecal ligation and puncture (CLP) model was applied to induce sepsis. Rats were separated into six groups with nine animals in each group, including healthy control, SBR, CLP, and CLP + SBR (5, 10, and 20 mg/kg) groups. Superoxide dismutase (SOD), glutathione (GSH) enzyme activities, and malondialdehyde (MDA) level were measured via enzyme-linked immunosorbent assay (ELISA). The messenger RNA (mRNA) expressions of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were evaluated by real-time polymerase chain reaction (RT-PCR). Histopathological changes in the lungs were investigated with hematoxylin and eosin (H&E). Results MDA levels and GSH and SOD enzyme activities were negatively affected in the CLP group, but SBR treatment ameliorated these oxidative stress parameters in the SBR1-3 groups (p< 0.05). The mRNA expressions of TNF-α and IL-1β were increased in the CLP group, and SBR treatment decreased those expression levels in a dose-dependent manner (p < 0.05). Organ damage and necrosis were seen in the CLP group and were alleviated in the SBR3 group. Immunohistochemical (IHC) analysis of lung tissues demonstrated decreased TNF-α and IL-1β immunopositivity in the SBR1-3 groups (p< 0.05). Conclusions SBR ameliorated sepsis-related lung injury in a dose-dependent manner. This compound has significant potential as a future agent in the treatment of sepsis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0894-1939 1521-0553 |
| Relation: | http://dx.doi.org/10.1080/08941939.2022.2136802; https://doaj.org/toc/0894-1939; https://doaj.org/toc/1521-0553; https://doaj.org/article/0438b7240c424ba89341cc9ff2fbb5bf |
| DOI: | 10.1080/08941939.2022.2136802 |
| الاتاحة: | https://doi.org/10.1080/08941939.2022.2136802 https://doaj.org/article/0438b7240c424ba89341cc9ff2fbb5bf |
| رقم الانضمام: | edsbas.23BD41A3 |
| قاعدة البيانات: | BASE |
| تدمد: | 08941939 15210553 |
|---|---|
| DOI: | 10.1080/08941939.2022.2136802 |