Academic Journal
Cloning and sequencing of the gene coding for the large subunit of methylamine dehydrogenase from Thiobacillus versutus
| العنوان: | Cloning and sequencing of the gene coding for the large subunit of methylamine dehydrogenase from Thiobacillus versutus |
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| المؤلفون: | Huitema, F, van Beeumen, J, van Driessche, G, Duine, J A, Canters, G W |
| المصدر: | Journal of Bacteriology ; volume 175, issue 19, page 6254-6259 ; ISSN 0021-9193 1098-5530 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 1993 |
| الوصف: | The gene that codes for the alpha-subunit of methylamine dehydrogenase from Thiobacillus versutus, madA, was cloned and sequenced. It codes for a protein of 395 amino acids preceded by a leader sequence of 31 amino acids. The derived amino acid sequence was confirmed by partial amino acid sequencing. The start of the mature protein could not be determined by direct sequencing, since the N terminus appeared to be blocked. Instead, it was determined by electrospray mass spectrometry. Confirmation of the results was obtained by sequencing the N terminus after pyroglutamate aminopeptidase digestion. The sequence is homologous to the Paracoccus denitrificans nucleotide sequence. A second open reading frame, called open reading frame 3, is located immediately downstream of madA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jb.175.19.6254-6259.1993 |
| الاتاحة: | http://dx.doi.org/10.1128/jb.175.19.6254-6259.1993 https://journals.asm.org/doi/pdf/10.1128/jb.175.19.6254-6259.1993 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.23A52E2C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jb.175.19.6254-6259.1993 |
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