Academic Journal
Fine physical and genetic mapping of powdery mildew resistance gene MlIW172 originating from wild emmer (Triticum dicoccoides)
| العنوان: | Fine physical and genetic mapping of powdery mildew resistance gene MlIW172 originating from wild emmer (Triticum dicoccoides) |
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| المؤلفون: | Ouyang, S., Zhang, D., Han, J., Zhao, X., Cui, Y., Song, W., Huo, N., Liang, Y., Xie, J., Wang, Z., Wu, Q., Chen, Y-X, Lu, P., Zhang, D-Y, Wang, L., Sun, H., Yang, T., Keeble-Gagnère, G., Appels, R., Doležel, J., Ling, H-Q, Luo, M., Gu, Y., Sun, Q., Liu, Z. |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2014 |
| الوصف: | Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 19326203 |
| Relation: | ispartof: PLoS ONE issue 6 vol 9; WOS:000338917900029; http://dx.doi.org/10.1371/journal.pone.0100160; 991005545235207891; https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Fine-physical-and-genetic-mapping-of/991005545235207891; https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12136600440007891/13137038730007891; alma:61MUN_INST/bibs/991005545235207891 |
| DOI: | 10.1371/journal.pone.0100160 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0100160 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Fine-physical-and-genetic-mapping-of/991005545235207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12136600440007891/13137038730007891 |
| Rights: | © 2014 Ouyang et al ; Open ; CC0 V1.0 |
| رقم الانضمام: | edsbas.22E60325 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0100160 |