Academic Journal
Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis
| العنوان: | Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis |
|---|---|
| المؤلفون: | Cancelliere R., Cosio T., Campione E., Corvino M., D’Amico M. P., Micheli L., Signori E., Contini G. |
| المساهمون: | Cancelliere, R, Cosio, T, Campione, E, Corvino, M, D’Amico, Mp, Micheli, L, Signori, E, Contini, G |
| بيانات النشر: | FRONTIERS MEDIA SA |
| سنة النشر: | 2023 |
| المجموعة: | Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
| مصطلحات موضوعية: | biochar, interleukin-6, point-ofcare, psoriasis, sandwich-based immunosensor, serum samples, Settore CHIM/01 |
| الوصف: | interleukin-6 (IL-6) plays a crucial role in autoimmunity and chronic inflammation. this study aims to develop a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical point-of-care device for the rapid detection of IL-6 in patients with psoriasis. precisely, a sandwich-based format immunosensor was developed using two primary antibodies (mAb-IL6 clone-5 and clone-7) and screen-printed electrodes modified with an inexpensive recycling electrochemical enhancing material, called biochar. mAb-IL6 clone-5 was used as a covalently immobilized capture bioreceptor on modified electrodes, and mAb-IL6 clone-7 was used to recognize the immunocomplex (Anti-IL6 clone-5 and IL-6) and form the sandwich. cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to conduct electrochemical characterization of the layer-by-layer assembly of the immunosensor, while square wave voltammetry (SWV) was used to perform the sensing. the developed immunosensor demonstrated robust analytical performance in buffer solution, with a wide linear range (LR) by varying from 2 to 250 pg/mL, a good limit of detection (LOD) of 0.78 pg/mL and reproducibility (RSD<7%). In addition, a spectrophotometric ELISA kit was employed to validate the results obtained with the label-free device by analyzing twenty-five serum samples from control and patients affected by psoriasis. a strong correlation in terms of pg/mL concentration of IL-6 was found comparing the two methods, with the advantage for our label-free biosensor of an ease use and a quicker detection time. based on IL-6 levels, the proposed immunosensor is a dependable, non-invasive screening device capable of predicting disease onset, progression, and treatment efficacy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38025060; info:eu-repo/semantics/altIdentifier/wos/WOS:001109915100001; volume:11; firstpage:1251360; journal:FRONTIERS IN CHEMISTRY; https://hdl.handle.net/2108/347024; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85177641716 |
| DOI: | 10.3389/fchem.2023.1251360 |
| الاتاحة: | https://hdl.handle.net/2108/347024 https://doi.org/10.3389/fchem.2023.1251360 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.22A70BF8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fchem.2023.1251360 |
|---|