Academic Journal
A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
| العنوان: | A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines |
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| المؤلفون: | Josep Escrivá-Fernández, Cristina Cueto-Ureña, Amalia Solana-Orts, Elisa Lledó, Begoña Ballester-Lurbe, Enric Poch |
| المصدر: | Journal of Biological Engineering, Vol 17, Iss 1, Pp 1-10 (2023) |
| بيانات النشر: | BMC |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Multiple myeloma, RND3/Rnd3, CRISPRi, CRISPR/Cas9, Lentiviral transduction, Biology (General), QH301-705.5 |
| الوصف: | Background Multiple myeloma (MM) is the second most common hematologic neoplasm which is characterized by proliferation and infiltration of plasmatic cells in the bone marrow. Currently, MM is considered incurable due to resistance to treatment. The CRISPR/Cas9 system has emerged as a powerful tool for understanding the role of different genetic alterations in the pathogenesis of hematologic malignancies in both cell lines and mouse models. Despite current advances of gene editing tools, the use of CRISPR/Cas9 technology for gene editing of MM have not so far been extended. In this work, we want to repress Rnd3 expression, an atypical Rho GTPase involved in several cellular processes, in MM cell lines using a CRISPR interference strategy. Results We have designed different guide RNAs and cloning them into a lentiviral plasmid, which contains all the machinery necessary for developing the CRISPR interference strategy. We co-transfected the HEK 293T cells with this lentiviral plasmid and 3rd generation lentiviral envelope and packaging plasmids to produce lentiviral particles. The lentiviral particles were used to transduce two different multiple myeloma cell lines, RPMI 8226 and JJN3, and downregulate Rnd3 expression. Additionally, the impact of Rnd3 expression absence was analyzed by a transcriptomic analysis consisting of 3’ UTR RNA sequencing. The Rnd3 knock-down cells showed a different transcriptomic profile in comparison to control cells. Conclusions We have developed a CRISPR interference strategy to generate stable Rnd3 knockdown MM cell lines by lentiviral transduction. We have evaluated this strategy in two MM cell lines, and we have demonstrated that Rnd3 silencing works both at transcriptional and protein level. Therefore, we propose CRISPR interference strategy as an alternative tool to silence gene expression in MM cell lines. Furthermore, Rnd3 silencing produces changes in the cellular transcriptomic profile. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1754-1611 |
| Relation: | https://doi.org/10.1186/s13036-023-00347-7; https://doaj.org/toc/1754-1611; https://doaj.org/article/1cf242b5330d4ca3a340f5e3ed54cb37 |
| DOI: | 10.1186/s13036-023-00347-7 |
| الاتاحة: | https://doi.org/10.1186/s13036-023-00347-7 https://doaj.org/article/1cf242b5330d4ca3a340f5e3ed54cb37 |
| رقم الانضمام: | edsbas.2277D37A |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17541611 |
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| DOI: | 10.1186/s13036-023-00347-7 |