التفاصيل البيبلوغرافية
| العنوان: |
Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy |
| المؤلفون: |
Wozei, E., Hermanowicz, S. W., Holman, H-Y. N. |
| المساهمون: |
United States. Department of Energy. Office of Biological and Environmental Research., University of California Center for Water Resources Project |
| المصدر: |
Journal Name: Biosensors and Bioelectronics; Journal Volume: 21; Journal Issue: 8; Related Information: Journal Publication Date: 02/15/2006 |
| بيانات النشر: |
Lawrence Berkeley National Laboratory |
| سنة النشر: |
2005 |
| المجموعة: |
University of North Texas: UNT Digital Library |
| مصطلحات موضوعية: |
Monitoring, Enzymes, Genes, Fluorescence, Yeasts, Fluorescein, 59 Basic Biological Sciences, Production, Water, 54 Environmental Sciences, Strains, Microscopy, Estrogens, Substrates |
| الوصف: |
Using a fluorescein di-{beta}-D-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17 {beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 minutes of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
Text |
| اللغة: |
English |
| Relation: |
rep-no: LBNL--58194; grantno: DE-AC02-05CH11231; osti: 882087; grantno: W-944; https://digital.library.unt.edu/ark:/67531/metadc873517/; ark: ark:/67531/metadc873517 |
| الاتاحة: |
https://digital.library.unt.edu/ark:/67531/metadc873517/ |
| رقم الانضمام: |
edsbas.21B24BCA |
| قاعدة البيانات: |
BASE |