Academic Journal
Development of a Method for Detection of SARS-CoV-2 Nucleocapsid Antibodies on Dried Blood Spot by DELFIA Immunoassay
| العنوان: | Development of a Method for Detection of SARS-CoV-2 Nucleocapsid Antibodies on Dried Blood Spot by DELFIA Immunoassay |
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| المؤلفون: | Damiani, Verena, Pizzinato, Erika, Cicalini, Ilaria, Demattia, Gianmaria, Zucchelli, Mirco, Natale, Luca, Palmarini, Claudia, Di Marzio, Claudia, Federici, Luca, De Laurenzi, Vincenzo, Pieragostino, Damiana |
| المساهمون: | Damiani, Verena, Pizzinato, Erika, Cicalini, Ilaria, Demattia, Gianmaria, Zucchelli, Mirco, Natale, Luca, Palmarini, Claudia, Di Marzio, Claudia, Federici, Luca, De Laurenzi, Vincenzo, Pieragostino, Damiana |
| سنة النشر: | 2023 |
| المجموعة: | ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| مصطلحات موضوعية: | DELFIA, ELISA, SARS-CoV-2, dried blood spot, nucleocapsid antibodies |
| الوصف: | Antibodies against the SARS-CoV-2 nucleocapsid protein are produced by the immune system in response to SARS-CoV-2 infection, but most available vaccines developed to fight the pandemic spread target the SARS-CoV-2 spike protein. The aim of this study was to improve the detection of antibodies against the SARS-CoV-2 nucleocapsid by providing a simple and robust method applicable to a large population. For this purpose, we developed a DELFIA immunoassay on dried blood spots (DBSs) by converting a commercially available IVD ELISA assay. A total of forty-seven paired plasma and dried blood spots were collected from vaccinated and/or previously SARS-CoV-2-infected subjects. The DBS-DELFIA resulted in a wider dynamic range and higher sensitivity for detecting antibodies against the SARS-CoV-2 nucleocapsid. Moreover, the DBS-DELFIA showed a good total intra-assay coefficient of variability of 14.6%. Finally, a strong correlation was found between SARS-CoV-2 nucleocapsid antibodies detected by the DBS-DELFIA and ELISA immunoassays (r = 0.9). Therefore, the association of dried blood sampling with DELFIA technology may provide an easier, minimally invasive, and accurate measurement of SARS-CoV-2 nucleocapsid antibodies in previously SARS-CoV-2-infected subjects. In conclusion, these results justify further research to develop a certified IVD DBS-DELFIA assay for detecting SARS-CoV-2 nucleocapsid antibodies useful for diagnostics as well as for serosurveillance studies. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/36900041; info:eu-repo/semantics/altIdentifier/wos/WOS:000947409400001; volume:13; issue:5; firstpage:897; journal:DIAGNOSTICS; https://hdl.handle.net/11564/805072; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85149788546; https://www.mdpi.com/2075-4418/13/5/897 |
| DOI: | 10.3390/diagnostics13050897 |
| الاتاحة: | https://hdl.handle.net/11564/805072 https://doi.org/10.3390/diagnostics13050897 https://www.mdpi.com/2075-4418/13/5/897 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2186E1EB |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/diagnostics13050897 |
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