Academic Journal
Higher β cell death in pregnant women, measured by DNA methylation patterns of cell-free DNA, compared to new-onset type 1 and type 2 diabetes subjects: a cross-sectional study
| العنوان: | Higher β cell death in pregnant women, measured by DNA methylation patterns of cell-free DNA, compared to new-onset type 1 and type 2 diabetes subjects: a cross-sectional study |
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| المؤلفون: | Teresa María Linares-Pineda, Carolina Gutiérrez-Repiso, Nerea Peña-Montero, María Molina-Vega, Fuensanta Lima Rubio, María Suárez Arana, Francisco J. Tinahones, María José Picón-César, Sonsoles Morcillo |
| المصدر: | Diabetology & Metabolic Syndrome, Vol 15, Iss 1, Pp 1-8 (2023) |
| بيانات النشر: | BMC |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Cell-free DNA, Biomarker, Gestational diabetes mellitus (GDM), Type 2 diabetes mellitus (T2DM), Type 1 diabetes mellitus (T1DM), β cell death, Nutritional diseases. Deficiency diseases, RC620-627 |
| الوصف: | Diabetes is a metabolic disorder of glucose homeostasis in which β cell destruction occurs silently and is detected mainly when symptoms appear. In the last few years, it has emerged a great interest in developing markers capable of detecting pancreatic β cell death focused on improving early diagnosis and getting a better treatment response, mainly in type 1 diabetes. But other types of diabetes would also benefit from early detection of β cell death. Differentially methylated circulating DNA is being studied as minimally invasive biomarker of cell death. We aimed to explore whether the unmethylated/methylated ratio of the insulin and amylin genes might be a good biomarker of β cell death in different types of diabetes. A lower index ∆Ct indicates a higher rate of β-cell death. Plasma samples from subjects without diabetes, pregnant women, pregnant with gestational diabetes (GDM), type 1 diabetes and type 2 diabetes were analyzed. A qPCR reaction with specific primers for both methylated and unmethylated fragments of insulin and amylin genes were carried out. Pregnant women, GDM and non- GDM, showed a higher β-cell death for both markers (∆INS = 3.8 ± 2.1 and ∆Amylin = 8.5 ± 3.6), whereas T1D presented lower rate (∆INS = 6.2 ± 2.1 and ∆Amylin = 10.7 ± 2.9) comparable to healthy subjects. The insulin methylation index was associated with the newborn birth weight (r = 0.46; p = 0.033) and with insulin resistance (r = -0.533; p = 0.027) in the GDM group. The higher rate of β-cell death was observed in pregnant women independently of their metabolic status. These indexes could be a good indicator of β cell death in processes caused by defects on insulin secretion, insulin action, or both. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1758-5996 |
| Relation: | https://doi.org/10.1186/s13098-023-01096-9; https://doaj.org/toc/1758-5996; https://doaj.org/article/238d7558ded64ae2a510102d16e16084 |
| DOI: | 10.1186/s13098-023-01096-9 |
| الاتاحة: | https://doi.org/10.1186/s13098-023-01096-9 https://doaj.org/article/238d7558ded64ae2a510102d16e16084 |
| رقم الانضمام: | edsbas.218426D9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17585996 |
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| DOI: | 10.1186/s13098-023-01096-9 |