Academic Journal
Comparative performance data for multiplex SARS-CoV-2 serological assays from a large panel of dried blood spot specimens
| العنوان: | Comparative performance data for multiplex SARS-CoV-2 serological assays from a large panel of dried blood spot specimens |
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| المؤلفون: | Cholette, François, Fabia, Rissa, Harris, Angela, Ellis, Hannah, Cachero, Karla, Schroeder, Lukas, Mesa, Christine, Lacap, Philip, Arnold, Corey, Galipeau, Yannick, Langlois, Marc-André, Colwill, Karen, Gingras, Anne-Claude, McGeer, Allison, Giles, Elizabeth, Day, Jacqueline, Osiowy, Carla, Durocher, Yves, Hankins, Catherine, Mazer, Bruce, Drebot, Michael, Kim, John |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | National Research Council Canada: NRC Publications Archive |
| مصطلحات موضوعية: | SARS-CoV-2, Covid-19, dried blood spots, serology, immunoassays, serosurveys |
| الوصف: | The extent of the COVID-19 pandemic will be better understood through serosurveys and SARS-CoV-2 antibody testing. Dried blood spot (DBS) samples will play a central role in large scale serosurveillance by simplifying biological specimen collection and transportation, especially in Canada. Direct comparative performance data on multiplex SARS-CoV-2 assays resulting from identical DBS samples are currently lacking. In our study, we aimed to provide performance data for the BioPlex 2200 SARS-CoV-2 IgG (Bio-Rad), V-PLEX SARS-CoV-2 Panel 2 IgG (MSD), and Elecsys Anti-SARS-CoV-2 (Roche) commercial assays, as well as for two highly scalable in-house assays (University of Ottawa and Mount Sinai Hospital protocols) to assess their suitability for DBS-based SARS-CoV-2 DBS serosurveillance. These assays were evaluated against identical panels of DBS samples collected from convalescent COVID-19 patients (n = 97) and individuals undergoing routine sexually transmitted and bloodborne infection (STBBI) testing prior to the COVID-19 pandemic (n = 90). Our findings suggest that several assays are suitable for serosurveillance (sensitivity >97% and specificity >98%). In contrast to other reports, we did not observe an improvement in performance using multiple antigen consensus-based rules to establish overall seropositivity. This may be due to our DBS panel which consisted of samples collected from convalescent COVID-19 patients with significant anti-spike, -receptor binding domain (RBD), and -nucleocapsid antibody titers. This study demonstrates that biological specimens collected as DBS coupled with one of several readily available assays are useful for large-scale COVID-19 serosurveillance. ; Peer reviewed: Yes ; NRC publication: Yes |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | Heliyon, Volume: 8, Issue: 9, Publication date: 2022-08-28; pii:S2405844022015584 |
| DOI: | 10.1016/j.heliyon.2022.e10270 |
| الاتاحة: | https://doi.org/10.1016/j.heliyon.2022.e10270 https://nrc-publications.canada.ca/eng/view/ft/?id=bc2882dc-9dff-4298-8e91-a7996502b335 https://nrc-publications.canada.ca/eng/view/object/?id=bc2882dc-9dff-4298-8e91-a7996502b335 https://nrc-publications.canada.ca/fra/voir/objet/?id=bc2882dc-9dff-4298-8e91-a7996502b335 |
| Rights: | Creative Commons, Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) (https://creativecommons.org/licenses/by-nc-nd/4.0/) ; Creative Commons, Attribution - Pas d’Utilisation Commerciale – Pas de Modification 4.0 International (CC BY-NC-ND 4.0) (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr) |
| رقم الانضمام: | edsbas.2140EBFA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2022.e10270 |