Academic Journal
Lipopolysaccharide activation of human monocytes mediated by CD14, results in a coordinated synthesis of tissue factor, TNF-α and IL-6
| العنوان: | Lipopolysaccharide activation of human monocytes mediated by CD14, results in a coordinated synthesis of tissue factor, TNF-α and IL-6 |
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| المؤلفون: | Osnes, L.T.N., Westvik, Å.B., Øvstebø, R., Joø, G-B., Okkenhaug, C., Kierulf, P. |
| المصدر: | Journal of Endotoxin Research ; volume 2, issue 1, page 27-35 ; ISSN 0968-0519 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 1995 |
| الوصف: | We have investigated the role of CD14 in lipopolysaccharide (LPS) stimulation of monocytes to tissue factor (TF) synthesis, tumor necrosis factor (TNF-α) antigen and interleukin-6 (IL-6) antigen release. Elutriation-purified monocytes or peripheral blood mononuclear cells (PBMC) preincubated with a blocking monoclonal antibody against CD14, and monocytes with a reduced expression of CD14 (phosphatidyl inositol specific phospholipase C (PI-PLC) treated monocytes and PBMC isolated from a patient suffering from paroxysmal nocturnal hemoglobinuria, PNH), were stimulated with increasing LPS concentrations. TNF-α antigen and IL-6 antigen were measured in the supernatants, and the cells harvested for measurement of procoagulant activity (PCA) as well as TF, TNF-α and IL-6 mRNA. We conclude that LPS stimulation of monocytes to TF, TNF-α and IL-6 synthesis as well as PCA is totally dependent on CD14 at clinically relevant LPS concentrations (1-50 ng/ml). Moreover, we demonstrate a close correlation between induction of PCA and release of TNF-a and IL-6 antigen indicating a common signalling pathway of induction of these mediators. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/096805199500200104 |
| الاتاحة: | https://doi.org/10.1177/096805199500200104 https://journals.sagepub.com/doi/pdf/10.1177/096805199500200104 |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.20FFE521 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/096805199500200104 |
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