Dissertation/ Thesis
Synchrotron X-ray fluorescence and super resolution correlative nanoimaging of metals and proteins in synapses of hippocampal neurons ; Nano-imagerie corrélative de fluorescence X synchrotron et de super résolution des métaux et des protéines dans les synapses de neurones d’hippocampe
| العنوان: | Synchrotron X-ray fluorescence and super resolution correlative nanoimaging of metals and proteins in synapses of hippocampal neurons ; Nano-imagerie corrélative de fluorescence X synchrotron et de super résolution des métaux et des protéines dans les synapses de neurones d’hippocampe |
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| المؤلفون: | Domart, Florelle |
| المساهمون: | Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux, Richard Ortega, Daniel Choquet |
| المصدر: | https://theses.hal.science/tel-02363022 ; Chimie analytique. Université de Bordeaux, 2019. Français. ⟨NNT : 2019BORD0185⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2019 |
| المجموعة: | HAL-IN2P3 (Institut national de physique nucléaire et de physique des particules) |
| مصطلحات موضوعية: | Analytical chemistry, Neurobiology, Trace metals, Synchrotron, Synapse, Correlative microscopy, Chimie analytique, Neurobiologie, Métaux traces, Microscopie corrélative, [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
| الوصف: | Metallic chemical elements such as Fe, Cu or Zn are present in minute quantities in the brain. The role of these trace metals in neuronal functions such as synaptic transmission and memory processes remains largely unclear. Moreover, metal dyshomeostasis is found in many neuropathologies, such as Alzheimer’s disease, Parkinson’s or amyotrophic lateral sclerosis. The interaction mechanisms of trace metals at the neuronal level are difficult to describe because of the lack of analytical methods with appropriate resolution and sensitivity to determine metal distribution at the synaptic level. Synchrotron X-ray fluorescence spectrometry (SXRF) is a multielemental chemical analysis method for describing the quantitative distribution of these elements at the sub-cellular scale with a nanometric resolution (40 nm at ESRF beamline ID16A ) and a very high sensitivity. In order to accurately interpret chemical imaging results, we have developed a protocol to correlate nano-SXRF imaging with stimulated emission depletion super resolution microscopy (STED), allowing the correlation of metal distribution and that of target proteins in super resolution. We labeled microtubules and F-actin of primary rat hippocampal neurons and imaged the cytoskeleton by STED microscopy before determining the distributions of P, S, Fe, Cu and Zn by SXRF spectrometry. We evidenced the colocalization of Zn and microtubules at the dendritic level and a localization of Cu mainly in the neck of dendritic spines rich in F-actin, and a distribution of Fe in the form of very localized points in the dendrites. These results highlight the crucial role of Zn in cytoskeleton architecture of hippocampal neurons and the developed correlative imaging method opens new perspectives for the study of metal dyshomeostasis in neurodegenerative diseases. ; Les éléments chimiques métalliques tels que Fe, Cu ou Zn sont présents en quantité infime dans le cerveau. Le rôle de ces métaux traces dans les fonctions neuronales, telles que la transmission synaptique et les ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| Relation: | NNT: 2019BORD0185; tel-02363022; https://theses.hal.science/tel-02363022; https://theses.hal.science/tel-02363022/document; https://theses.hal.science/tel-02363022/file/DOMART_FLORELLE_2019.pdf |
| الاتاحة: | https://theses.hal.science/tel-02363022 https://theses.hal.science/tel-02363022/document https://theses.hal.science/tel-02363022/file/DOMART_FLORELLE_2019.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.20EEAE17 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |