Academic Journal
MiR‐193b deregulation is associated with Parkinson's disease
| العنوان: | MiR‐193b deregulation is associated with Parkinson's disease |
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| المؤلفون: | Baghi, Masoud, Yadegari, Elaheh, Rostamian Delavar, Mahsa, Peymani, Maryam, Ganjalikhani‐Hakemi, Mazdak, Salari, Mehri, Nasr‐Esfahani, Mohammad Hossein, Megraw, Timothy L., Ghaedi, Kamran |
| المصدر: | Journal of Cellular and Molecular Medicine ; volume 25, issue 13, page 6348-6360 ; ISSN 1582-1838 1582-4934 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | PGC‐1α/FNDC5/BDNF has found to be a critical pathway in neurodegeneration. MicroRNAs (miR(NA)s) are non‐coding regulatory RNAs whose dysregulation has been observed in multiple neurological disorders, and miRNA‐mediated gene deregulation plays a decisive role in PD. Here, candidate miRNA was chosen based on the literature survey and in silico studies. Chronic and acute models of PD were created using MPP+‐treated SH‐SY5Y cells. Twenty PD patients and 20 healthy volunteers were recruited. RT‐qPCR was performed to assess the expression of miRNA and genes. Severe mitochondrial dysfunction induced by acute MPP+ treatment instigated compensatory mechanisms through enhancing expression of PGC‐1α/FNDC5/BDNF pathway genes, while chronic MPP+ toxicity led to down‐regulated levels of the genes in SH‐SY5Y cells. PD peripheral blood mononuclear cells (PBMCs) also showed decreased expression of target genes. There were significant changes in the level of miR‐193b in both models, as well as PD PBMCs. Moreover, miR‐193b overexpression significantly affected PGC‐1α, FNDC5 and TFAM levels. Interestingly, down‐regulations of PGC‐1α, FNDC5, BDNF and TFAM were inversely correlated with miR‐193b up‐regulation in PD PBMCs. This study showed the deregulation of PGC‐1α/FNDC5/BDNF pathway in PD models and PBMCs, verifying its importance in neurodegeneration. Our findings also revealed that miR‐193b functions in PD development, possibly through regulating PGC‐1α/FNDC5/BDNF pathway, suggesting miR‐193b as a potential biomarker for PD diagnosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jcmm.16612 |
| الاتاحة: | http://dx.doi.org/10.1111/jcmm.16612 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jcmm.16612 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jcmm.16612 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.20DB1906 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jcmm.16612 |
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