Academic Journal
Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide
| العنوان: | Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide |
|---|---|
| المؤلفون: | Durao, Paulo, Kis, Peter, Chelo, Ivo M, Ventura, M. Rita, Martins, Lígia O. |
| بيانات النشر: | Wiley |
| سنة النشر: | 2023 |
| المجموعة: | Universidade de Lisboa: repositório.UL |
| مصطلحات موضوعية: | antibiotics, CotA-laccase, enzymatic transformation, hydrogen peroxide, animal toxicity, Bioremediation |
| الوصف: | Antibiotics are micropollutants accumulating in our rivers and wastewaters, potentially leading to bacterial antibiotic resistance, a worldwide problem to which there is no current solution. Here, we have developed an environmentally friendly two-step process to transform the antibiotic rifampicin (RIF) into non-antimicrobial compounds. The process involves an enzymatic oxidation step by the bacterial CotA-laccase and a hydrogen peroxide bleaching step. NMR identified rifampicin quinone as the main product of the enzymatic oxidation. Growth of Escherichia coli strains in the presence of final degradation products (FP) and minimum inhibitory concentration (MIC) measurements confirmed that FP are non-anti-microbial compounds, and bioassays suggest that FP is not toxic to eukaryotic organisms. Moreover, competitive fitness assays between susceptible and RIF-resistant bacteria show that susceptible bacteria is strongly favoured in the presence of FP. Our results show that we have developed a robust and environmentally friendly process to effectively remediate rifampicin from antibiotic contaminated environments. ; info:eu-repo/semantics/acceptedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1439-4227 1439-7633 |
| Relation: | FCT 2021.00778.CEECIND; FCT UIDB/00329/2020; European Union’s Horizon Europe research and innovation programme under the Widening Participation and Spreading Excellence grant 101090282; FCT MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, 480UIDP/04612/2020); FCT LS4FUTURE Associated Laboratory (LA/P/0087/2020); Durao, Paulo, Kis, Peter, Chelo, Ivo M., Ventura, M. Rita, Martins, Lígia O., ChemBioChem 2023, e202300627.; http://hdl.handle.net/10451/60483 |
| DOI: | 10.1002/cbic.202300627 |
| الاتاحة: | http://hdl.handle.net/10451/60483 https://doi.org/10.1002/cbic.202300627 |
| Rights: | embargoedAccess |
| رقم الانضمام: | edsbas.20B8022E |
| قاعدة البيانات: | BASE |
| تدمد: | 14394227 14397633 |
|---|---|
| DOI: | 10.1002/cbic.202300627 |