Academic Journal
Genetic Characterization of Multidrug-Resistant Acinetobacter baumannii and Synergy Assessment of Antimicrobial Combinations
| العنوان: | Genetic Characterization of Multidrug-Resistant Acinetobacter baumannii and Synergy Assessment of Antimicrobial Combinations |
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| المؤلفون: | Aurora Luna-De-Alba, Samantha Flores-Treviño, Adrián Camacho-Ortiz, Juan Francisco Contreras-Cordero, Paola Bocanegra-Ibarias |
| المصدر: | Antibiotics, Vol 13, Iss 11, p 1079 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | genetic characterization, colistin, meropenem, efflux pumps, porins, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Background/Objectives : A. baumannii is a prominent nosocomial pathogen due to its drug-resistant phenotype, representing a public health problem. In this study, the aim was to determine the effect of different antimicrobial combinations against selected multidrug-resistant (MDR) or extensive drug-resistant (XDR) isolates of A. baumannii . Methods : MDR or XDR A. baumannii isolates were characterized by assessing genes associated with drug resistance, efflux pumps, porin expression, and biofilm formation. The activities of antimicrobial combinations including tigecycline, ampicillin/sulbactam, meropenem, levofloxacin, and colistin were evaluated using checkerboard and time-to-kill assays on isolates with different susceptibility profiles and genetic characteristics. Results : Genetic characterization of MDR/XDR strains ( n = 100) included analysis of OXA-24/40 gene carbapenemase (98%), genes encoding aminoglycoside-modifying enzymes (44%), and parC gene mutations (10%). AdeIJK, AdeABC, and AdeFGH efflux pumps were overexpressed in 17–34% of isolates. Omp33-36, OmpA, and CarO membrane porins were under-expressed in 50–76% of isolates; CarO was overexpressed in 22% of isolates. Isolates showed low biofilm production (11%). Synergistic activity was observed with levofloxacin-ampicillin/sulbactam and meropenem-colistin, which were able to inhibit bacterial growth. Conclusions : Genetic characteristics of A. baumannii were highly variable among the strains. Synergistic activity was observed with meropenem-colistin and levofloxacin-ampicillin/sulbactam combinations in the checkerboard method, but not in the time-to-kill assays. These discrepancies among both methods indicate that further studies are needed to determine the best therapeutic combination for treating infections by A. baumannii . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://www.mdpi.com/2079-6382/13/11/1079; https://doaj.org/toc/2079-6382; https://doaj.org/article/e2dc1007e586453681e9ddab96155612 |
| DOI: | 10.3390/antibiotics13111079 |
| الاتاحة: | https://doi.org/10.3390/antibiotics13111079 https://doaj.org/article/e2dc1007e586453681e9ddab96155612 |
| رقم الانضمام: | edsbas.2060224C |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/antibiotics13111079 |
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