Dissertation/ Thesis
Study of Volatile Organic Compounds toxicity at Air-Liquid Interface during repeated exposure : Case of toluene, its homologous and outflow emissions after its catalytic treatment ; Etude en Interface Air-Liquide de la toxicité des Composés Organiques Volatils lors d’expositions répétées : Cas du toluène, de ses homologues et des émissions issues de son traitement catalytique
| العنوان: | Study of Volatile Organic Compounds toxicity at Air-Liquid Interface during repeated exposure : Case of toluene, its homologous and outflow emissions after its catalytic treatment ; Etude en Interface Air-Liquide de la toxicité des Composés Organiques Volatils lors d’expositions répétées : Cas du toluène, de ses homologues et des émissions issues de son traitement catalytique |
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| المؤلفون: | Méausoone, Clémence |
| المساهمون: | Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale (ULCO), Université du Littoral Côte d'Opale, Dominique Courcot |
| المصدر: | https://theses.hal.science/tel-02887101 ; Médecine humaine et pathologie. Université du Littoral Côte d'Opale, 2019. Français. ⟨NNT : 2019DUNK0545⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Toxicology, Air-Liquid Interface Exposure, Volatile Organic Compounds, Repeated Exposures, Toxicologie, Exposition en Interface Air-liquide, Composés Organiques Volatils, Expositions répétées, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.TOX]Life Sciences [q-bio]/Toxicology |
| الوصف: | Toluene is a solvent widely used in manufacturing industries. It belongs to a family of volatile organic compounds (VOCs), many of which have adverse impacts on human health and are classified as carcinogenic, mutagenic or toxic for reproduction. Different measures have been implemented to reduce the emissions of toxic compounds, such as their replacement in the industry by less harmful compounds and/or reducing gas emissions at the source. In this context, the first objective of the research was to investigate the acute toxicity and the one after repeated exposure to toluene and its superior homologous solvents, which can be used as its substitution compounds, as well as its lower homologous on human bronchial epithelial cells using an air/liquid interface exposure device. The second objective was to assess the toxicity of gaseous effluents from the degradation of toluene by catalytic oxidation. For this purpose, BEAS-2B cells were exposed during 1 hour for 1, 3 or 5 days to benzene, toluene, xylene or mesitylene, and to the exhausts of catalytic oxidation of toluene. Toxic effects were evaluated through cytotoxicity, inflammatory response and gene expression of xenobiotic metabolism enzymes (XME). Exposure of BEAS-2B cells to toluene and its homologous compounds revealed the involvement of metabolic pathways specific to each compound. A significant increase in inflammatory marker response was also observed, with a higher concentration after cell exposure to benzene and to xylene compared to the other molecules. With regard to exposure to gaseous effluents from the catalytic oxidation of toluene, the late expression of genes involved in the metabolism of aromatic organic xenobiotics has made possible to highlight the presence of by-products, such as benzene or polycyclic aromatic hydrocarbons. In conclusion, the results obtained in this project show the interest of conducting repeated in vitro exposures to detect potential late effects, and the importance of toxicological validation of catalytic systems before ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| Relation: | NNT: 2019DUNK0545; tel-02887101; https://theses.hal.science/tel-02887101; https://theses.hal.science/tel-02887101/document; https://theses.hal.science/tel-02887101/file/these_Meausoone_Clemence.pdf |
| الاتاحة: | https://theses.hal.science/tel-02887101 https://theses.hal.science/tel-02887101/document https://theses.hal.science/tel-02887101/file/these_Meausoone_Clemence.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.1FD663FB |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |