Academic Journal
Capillarity‐Driven Enrichment and Hydrodynamic Trapping of Trace Nucleic Acids by Plasmonic Cavity Membrane for Rapid and Sensitive Detections
| العنوان: | Capillarity‐Driven Enrichment and Hydrodynamic Trapping of Trace Nucleic Acids by Plasmonic Cavity Membrane for Rapid and Sensitive Detections |
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| المؤلفون: | Whang, Keumrai, Min, Junwon, Shin, Yonghee, Hwang, Inhyeok, Lee, Hyunjoo, Kwak, Taejin, La, Ju A., Kim, Sungbong, Kim, Dongchoul, Lee, Luke P., Kang, Taewook |
| المساهمون: | Air Force Office of Scientific Research, National Research Foundation of Korea |
| المصدر: | Advanced Materials ; volume 36, issue 28 ; ISSN 0935-9648 1521-4095 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Small‐reactor‐based polymerase chain reaction (PCR) has attracted considerable attention. A significant number of tiny reactors must be prepared in parallel to capture, amplify, and accurately quantify few target genes in clinically relevant large volume, which, however, requires sophisticated microfabrication and longer sample‐to‐answer time. Here, single plasmonic cavity membrane is reported that not only enriches and captures few nucleic acids by taking advantage of both capillarity and hydrodynamic trapping but also quickly amplifies them for sensitive plasmonic detection. The plasmonic cavity membrane with few nanoliters in a void volume is fabricated by self‐assembling gold nanorods with SiO 2 tips. Simulations reveal that hydrodynamic stagnation between the SiO 2 tips is mainly responsible for the trapping of the nucleic acid in the membrane. Finally, it is shown that the plasmonic cavity membrane is capable of enriching severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) genes up to 20 000‐fold within 1 min, amplifying within 3 min, and detecting the trace genes as low as a single copy µL −1 . It is anticipated that this work not only expands the utility of PCR but also provides an innovative way of the enrichment and detection of trace biomolecules in a variety of point‐of‐care testing applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/adma.202403896 |
| الاتاحة: | http://dx.doi.org/10.1002/adma.202403896 https://onlinelibrary.wiley.com/doi/pdf/10.1002/adma.202403896 |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.1F6F6AC7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/adma.202403896 |
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