Academic Journal

Mass Spectrometry-Based Untargeted Proteomics for the Assessment of Food Authenticity: The Case of Farmed Versus Wild-Type Salmon.

التفاصيل البيبلوغرافية
العنوان: Mass Spectrometry-Based Untargeted Proteomics for the Assessment of Food Authenticity: The Case of Farmed Versus Wild-Type Salmon.
المؤلفون: Jens Brockmeyer, Ilario Losito, Marion Fresch, Giuseppina M Fiorino, Ina Brümmer, Linda Monaci, Marco Arlorio
سنة النشر: 2019
المجموعة: Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository
مصطلحات موضوعية: Pharmacology, Agronomy and Crop Science, Environmental Chemistry, Food Science, Analytical Chemistry
الوصف: Background: Omics technologies have been widely applied in different fields, among which, proteomics is gaining increasing interest for its application to the authenticity of food products. MS, typically coupled with LC, represents a key technique for proteomics-related studies dedicated to fish and other seafood products by using a bottom-up approach. Objective and Methods: In this paper, the optimization of an untargeted proteomics-based method using LC separation and MS detection relying on a quadrupole time-of-flight mass spectrometer is described and applied to the analysis of Canadian farmed and wild-type salmon, followed by statistical analysis based on principal component (PC) analysis. Results and Conclusions: This untargeted approach, using a data-independent acquisition MS scheme, demonstrated the ability to effectively discriminate salmon belonging to the two classes. Furthermore, selected peptides showing high loadings on PC1 could represent potential candidate peptide markers able to discriminate farmed from wild-type salmon samples in the future.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: url:https://www.openaccessrepository.it/communities/itmirror; https://www.openaccessrepository.it/record/101041
DOI: 10.1093/jaoac/102.5.1339
الاتاحة: https://www.openaccessrepository.it/record/101041
https://doi.org/10.1093/jaoac/102.5.1339
Rights: info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.1F65A567
قاعدة البيانات: BASE
الوصف
DOI:10.1093/jaoac/102.5.1339