Academic Journal

Repertoire of free-living protozoa in contact lens solutions

التفاصيل البيبلوغرافية
العنوان: Repertoire of free-living protozoa in contact lens solutions
المؤلفون: Bouchoucha, Ibtissem, Aziz, Aurore, Hoffart, Louis, Drancourt, Michel
المساهمون: Ophtalmologie Hôpital de la Timone - APMH, Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital de la Timone CHU - APHM (TIMONE), Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques - CNRS Biologie (INSB-CNRS)-Institut des sciences biologiques - CNRS Biologie (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)
المصدر: ISSN: 1471-2415 ; BMC Ophthalmology ; https://hal.science/hal-01455794 ; BMC Ophthalmology, 2016, 16, ⟨10.1186/s12886-016-0370-6⟩.
بيانات النشر: HAL CCSD
BioMed Central
سنة النشر: 2016
المجموعة: Aix-Marseille Université: HAL
مصطلحات موضوعية: Acanthamoeba, Keratitis, Contact lens solution, Protozoa, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
الوصف: International audience ; Background: The repertoire of free-living protozoa in contact lens solutions is poorly known despite the fact that such protozoa may act as direct pathogens and may harbor intra-cellular pathogens. Methods: Between 2009 and 2014, the contact lens solutions collected from patients presenting at our Ophthalmology Department for clinically suspected keratitis, were cultured on non-nutrient agar examined by microscope for the presence of free-living protozoa. All protozoa were identified by 18S rRNA gene sequencing. Results: A total of 20 of 233 (8.6 %) contact lens solution specimens collected from 16 patients were cultured. Acanthamoeba amoeba in 16 solutions (80 %) collected from 12 patients and Colpoda steini, Cercozoa sp., Protostelium sp. and a eukaryotic more closely related to Vermamoeba sp., were each isolated in one solution. Cercozoa sp., Colpoda sp., Protostelium sp. and Vermamoeba sp. are reported for the first time as contaminating contact lens solutions. Conclusion: The repertoire of protozoa in contact lens solutions is larger than previously known.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: hal-01455794; https://hal.science/hal-01455794; https://hal.science/hal-01455794/document; https://hal.science/hal-01455794/file/12886_2016_Article_370.pdf; PUBMEDCENTRAL: PMC5086047
DOI: 10.1186/s12886-016-0370-6
الاتاحة: https://hal.science/hal-01455794
https://hal.science/hal-01455794/document
https://hal.science/hal-01455794/file/12886_2016_Article_370.pdf
https://doi.org/10.1186/s12886-016-0370-6
Rights: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
رقم الانضمام: edsbas.1F4BE2D6
قاعدة البيانات: BASE
الوصف
DOI:10.1186/s12886-016-0370-6