Academic Journal
Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein
| العنوان: | Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein |
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| المؤلفون: | Zarena, A. S., Shubha Gopal, Vineeth, R. |
| سنة النشر: | 2014 |
| المجموعة: | University of Mysore: ePrints@UoM |
| مصطلحات موضوعية: | Microbiology |
| الوصف: | In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is â��29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 M, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 M in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200 g/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | http://eprints.uni-mysore.ac.in/4323/1/Mic_2014_Shubha%20Gopal_02.pdf; Zarena, A. S. and Shubha Gopal and Vineeth, R. (2014) Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein. Journal of Analytical Methods in Chemistry, 2014. |
| DOI: | 10.1155/2014/989543 |
| الاتاحة: | http://eprints.uni-mysore.ac.in/4323/ http://eprints.uni-mysore.ac.in/4323/1/Mic_2014_Shubha%20Gopal_02.pdf https://doi.org/10.1155/2014/989543 |
| رقم الانضمام: | edsbas.1F2177E7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2014/989543 |
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