Academic Journal
Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures
| العنوان: | Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures |
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| المؤلفون: | Consuegra González, Cesar, Crespo Castejón, Francisco, Dorado Martín, Jesús, María Díaz Jiménez, Sánchez Calabuig, María Jesús, Beltrán Breña, Paula, Pérez Cerezales, Serafín, Rizos, Dimitri, Hidalgo Prieto |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Universidad Complutense de Madrid (UCM): E-Prints Complutense |
| مصطلحات موضوعية: | 636.09, Semen, Equine, cryopreservation, Fertility, Veterinaria, 24 Ciencias de la Vida |
| الوصف: | The aim of this study was to evaluate the fertilizing capacity of frozen or vitrified stallion sperm after assessing different warming procedures. In Experiment 1, different warming procedures were compared after sperm vitrification: immersion in extender at 43 ◦C (C), or in a water bath at 37 ◦C/30 s (W37), 43 ◦C/10 s (W43) or 60 ◦C/5 s (W60). With the W60 treatment, there were greater values (P < 0.05) for VCL (83.93 ± 3.6 μm/s) and ALH (3.00 ± 0.2 μm) than freezing and with the C group, and greater values (P < 0.001) for PM (35.33 ± 2.5 %) than with the W43 treatment. In Experiment 2, the fertilizing capacity of vitrified and frozen sperm was assessed utilizing heterologous IVF procedures, using cattle oocytes. Vitrification resulted in greater values (P < 0.05) than freezing for the number of bound sperm (1.36 ± 0.3 and 0.69 ± 0.2, respectively). There were no differences between frozen or vitrified sperm in pronuclear formation (26 hours post-insemination - hpi; 14.08 ± 4.2 % and 22.78 ± 4.8 %, respectively) or cleavage rate (32.77 ± 4.3 % and 39.66 ± 4.6 %, respectively). In conclusion, vitrified stallion sperm warmed in a water bath at 60 ºC had the capacity to penetrate cattle oocytes, leading to pronuclear formation and hybrid embryo cleavage after heterologous IVF. ; FPU Spanish fellowship (16/05745) from MECD (“Ministerio de Educaci´on, Cultura y Deporte”). ; Depto. de Medicina y Cirugía Animal ; Fac. de Veterinaria ; TRUE ; pub |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0378-4320 |
| Relation: | AGL-2013-42726-R; AGL2015-70140-R; https://hdl.handle.net/20.500.14352/97208; Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Sánchez-Calabuig MJ, Beltrán-Breña P, Pérez-Cerezales S, Rizos D, Hidalgo M. Fertilizing capacity of vitrified stallion sperm assessed utilizing heterologous IVF after different semen warming procedures. Anim Reprod Sci. 2020 Dec;223:106627; http://www.journals.elsevier.com/animal-reproduction-science/ |
| DOI: | 10.1016/j.anireprosci.2020.106627 |
| الاتاحة: | https://hdl.handle.net/20.500.14352/97208 https://doi.org/10.1016/j.anireprosci.2020.106627 http://www.journals.elsevier.com/animal-reproduction-science/ |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; open access |
| رقم الانضمام: | edsbas.1E6E8637 |
| قاعدة البيانات: | BASE |
| تدمد: | 03784320 |
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| DOI: | 10.1016/j.anireprosci.2020.106627 |