Academic Journal
Moderate conformational impact of citrate on ovotransferrin considerably increases its capacity to self-assemble at the interface
| العنوان: | Moderate conformational impact of citrate on ovotransferrin considerably increases its capacity to self-assemble at the interface |
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| المؤلفون: | Le Floch-Fouéré, Cécile, Pezennec, Stephane, Pasco, Maryvonne, Paboeuf, Gilles, Renault, Anne, Beaufils, Sylvie |
| المساهمون: | Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0021-9797. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2015 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | interface air-eau, ovotransferrine, adsorption, interaction protéine-ion, traitement thermique, protéine de l'oeuf, citrate, dénaturation, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| الوصف: | International audience ; We have compared the behavior of ovotransferrin at the air-solution interface in the presence of a monovalention (acetate), or a divalent ion (citrate), the latter being known to induce conformational changesof this protein upon interaction with its iron-binding sites. We have characterised the adsorption layer atthe air–water interface in terms of homogeneity, surface concentration excess and rheological propertiesat pH 4.0. Besides we have investigated the bulk conformation in the presence of the two anions. In thepresence of citrate only, interfacial layers display well-defined domains of higher overall surface concentrationsuggesting multilayers adsorption. Citrate also induces higher helical content and stabilizes theprotein against thermal denaturation. Hence we propose that these changes are involved in the propensityof ovotransferrin to self-assemble at the air–water interface resulting in thick and heterogeneousinterfacial layer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/25313487; hal-01128555; https://hal.science/hal-01128555; https://hal.science/hal-01128555/document; https://hal.science/hal-01128555/file/1-s2.0-S0021979714006845-main.pdf; PRODINRA: 275021; PUBMED: 25313487; WOS: 000345109000029 |
| DOI: | 10.1016/j.jcis.2014.09.035 |
| الاتاحة: | https://hal.science/hal-01128555 https://hal.science/hal-01128555/document https://hal.science/hal-01128555/file/1-s2.0-S0021979714006845-main.pdf https://doi.org/10.1016/j.jcis.2014.09.035 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.1E408D23 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jcis.2014.09.035 |
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