Academic Journal
The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent
| العنوان: | The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent |
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| المؤلفون: | Silva, C, Andrade, N, Guimaraes, JT, Cardoso, E, Meireles, C, Pinto, V, Paiva, J, Martel, F |
| المساهمون: | Instituto de Saúde Pública da Universidade do Porto |
| بيانات النشر: | Wiley |
| سنة النشر: | 2022 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| الوصف: | Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12ADMBA) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, 3H-DG uptake and lactic acid production in both cell lines. The stimulatory effects of insulin upon cell proliferation and 3H-DG uptake were hampered by rapamycin, LY294001 and BAY-876, in both cell lines. In conclusion, hyperinsulinemia, one important characteristic of T2DM, contributes to the initiation of breast cancer by a PI3K- and mTOR-dependent mechanism involving increased GLUT1-mediated glucose uptake. Significance: The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent. ; European Regional Development Fund, Grant/Award Number: NORTE-08-5369-FSE-000018; Fundação para a Ciência e a Tecnologia, Grant/Award Numbers: UID/BIM/04293/2013, UIDB/50006/2020 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0263-6484 1099-0844 |
| Relation: | Cell Biochem Funct. 2022 Mar;40(2):127-137.doi:10.1002/cbf.3681. Epub 2022 Jan 10.; https://hdl.handle.net/10216/154381 |
| DOI: | 10.1002/cbf.3681 |
| الاتاحة: | https://hdl.handle.net/10216/154381 https://doi.org/10.1002/cbf.3681 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.1DC74C6F |
| قاعدة البيانات: | BASE |
| تدمد: | 02636484 10990844 |
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| DOI: | 10.1002/cbf.3681 |