Academic Journal
Advancements in minimal residual disease detection: a practical approach using single-cell droplet PCR for comprehensive monitoring in hematological malignancy
| العنوان: | Advancements in minimal residual disease detection: a practical approach using single-cell droplet PCR for comprehensive monitoring in hematological malignancy |
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| المؤلفون: | Uchiyama, Satoshi, Fukushima, Kentaro, Katagiri, Seiichiro, Tsuchiya, Junichi, Kubo, Tomohiro, Chi, SungGi, Minami, Yosuke |
| المصدر: | Therapeutic Advances in Hematology ; volume 15 ; ISSN 2040-6207 2040-6215 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2024 |
| الوصف: | The identification of chromosomal abnormalities accompanied by copy number alterations is important for understanding tumor characteristics. Testing methodologies for copy number abnormality have limited sensitivity, resulting in their use only for the sample provided at the time of diagnosis or recurrence of malignancy, but not for the monitoring of minimal residual disease (MRD) during and after therapy. We developped the “DimShift” technology which enable to measure the copy number of target gene/chromosome in each cell, which is given by the single cell droplet PCR. Qualitative result of DimShift given by peripheral blood was perfectly concordant with that of bone marrow. These findings and performances are promising to be the new methodology for MRD detection in malignant diseases utilizing bone marrow as well as peripheral blood. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/20406207241245510 |
| الاتاحة: | http://dx.doi.org/10.1177/20406207241245510 https://journals.sagepub.com/doi/pdf/10.1177/20406207241245510 https://journals.sagepub.com/doi/full-xml/10.1177/20406207241245510 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.1DBDF97D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/20406207241245510 |
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