التفاصيل البيبلوغرافية
| العنوان: |
SEC analysis of recombinant proteins. |
| المؤلفون: |
Peter Naniima (114016), Eleonora Naimo (11648366), Sandra Koch (3944867), Ute Curth (8815), Khaled R. Alkharsah (10725016), Luisa J. Ströh (11648369), Anne Binz (309492), Jan-Marc Beneke (11648372), Benjamin Vollmer (6843044), Heike Böning (11648375), Eva Maria Borst (11648378), Prashant Desai (615738), Jens Bohne (7271), Martin Messerle (139659), Rudolf Bauerfeind (152093), Pierre Legrand (196881), Beate Sodeik (191751), Thomas F. Schulz (8355900), Thomas Krey (171661) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Molecular Biology, Cancer, Infectious Diseases, Virology, striking structural similarity, 6 &# 197, &# 945, 12 capsid vertices, capsid require, capsid association, results pave, provide insights, portal channel, portal cap, infectious progeny, infected cells, immunocompromised patients, genome packaging, better understand, also present |
| الوصف: |
(A–D) Elution profiles from a Superdex 200 Increase 10/300 GL size exclusion column obtained upon purification of individual CTDs from HCMV pUL77 (A) , KSHV pORF19 (B) , and MuHV-68 pORF19 (C) . In all cases, the majority of the protein elutes at a volume presumably corresponding to a monomer. (D ) Elution profile of oxidized pORF19 KCTD CC analyzed by SEC aligned to the profile of the non-oxidized wt pORF19 KCTD . The vertical red dotted lines mark the elution volume of the oxidized pORF19 KCTD CC and the non-oxidized wt pORF19 KCTD (B) . The oxidized protein elutes at an earlier volume, underlining the difference in oligomeric state. The underlying data for all chromatograms can be found in S3 Data . CTD, carboxyl-terminal domain; HCMV, human cytomegalovirus; KSHV, Kaposi’s sarcoma-associated herpesvirus; MuHV-68, murid gammaherpesvirus 68; SEC, size exclusion chromatography; wt, wild-type. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/SEC_analysis_of_recombinant_proteins_/16931051 |
| DOI: |
10.1371/journal.pbio.3001423.s002 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001423.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.1DA33FD2 |
| قاعدة البيانات: |
BASE |