Academic Journal
Heterodimerization of UNC-13/RIM regulates synaptic vesicle release probability but not priming in C. elegans
| العنوان: | Heterodimerization of UNC-13/RIM regulates synaptic vesicle release probability but not priming in C. elegans |
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| المؤلفون: | Haowen Liu, Lei Li, Daniel Nedelcu, Qi Hall, Lijun Zhou, Wei Wang, Yi Yu, Joshua M Kaplan, Zhitao Hu |
| المصدر: | eLife, Vol 8 (2019) |
| بيانات النشر: | eLife Sciences Publications Ltd |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | synaptic transmission, neuromuscular junction, release probability, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | UNC-13 proteins play an essential role in synaptic transmission by recruiting synaptic vesicles (SVs) to become available for release, which is termed SV priming. Here we show that the C2A domain of UNC-13L, like the corresponding domain in mammalian Munc13-1, displays two conserved binding modes: forming C2A/C2A homodimers, or forming a heterodimer with the zinc finger domain of UNC-10/RIM (C2A/RIM). Functional analysis revealed that UNC-13L’s C2A promotes synaptic transmission by regulating a post-priming process. Stimulus-evoked release but not SV priming, was impaired in unc-10 mutants deficient for C2A/RIM heterodimerization, leading to decreased release probability. Disrupting C2A/C2A homodimerization in UNC-13L-rescued animals had no effect on synaptic transmission, but fully restored the evoked release and the release probability of unc-10/RIM mutants deficient for C2A/RIM heterodimerization. Thus, our results support the model that RIM binding C2A releases UNC-13L from an autoinhibitory homodimeric complex to become fusion-competent by functioning as a switch only. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/40585; https://doaj.org/toc/2050-084X; e40585; https://doaj.org/article/53f62d9fca2e46efb7c4a1e3059644ca |
| DOI: | 10.7554/eLife.40585 |
| الاتاحة: | https://doi.org/10.7554/eLife.40585 https://doaj.org/article/53f62d9fca2e46efb7c4a1e3059644ca |
| رقم الانضمام: | edsbas.1D90A5B8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.40585 |