Academic Journal
Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis
| العنوان: | Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis |
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| المؤلفون: | Ramirez A. B., Bhat R., Sahay D., De Angelis C., Thangavel H., Hedayatpour S., Dobrolecki L. E., Nardone A., Giuliano M., Nagi C., Rimawi M., Osborne C. K., Lewis M. T., Stilwell J. L., Kaldjian E. P., Schiff R., Trivedi M. V. |
| المساهمون: | Ramirez, A. B., Bhat, R., Sahay, D., De Angelis, C., Thangavel, H., Hedayatpour, S., Dobrolecki, L. E., Nardone, A., Giuliano, M., KHALIL RODRIGUEZ, Nagi, Rimawi, M., Osborne, C. K., Lewis, M. T., Stilwell, J. L., Kaldjian, E. P., Schiff, R., Trivedi, M. V. |
| سنة النشر: | 2019 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | Breast cancer, Chemotherapy, Circulating tumor cell, Patient-derived xenograft, Single-cell analysi, Animal, Antineoplastic Agent, Biomarkers, Tumor, Breast Neoplasm, Cell Line, Cell Separation, Class I Phosphatidylinositol 3-Kinase, Female, Human, Keratin, Leukocyte Common Antigen, Mice, SCID, Mutation, Neoplasm Transplantation, Neoplastic Cells, Circulating, Sequence Analysis, DNA, Single-Cell Analysis |
| الوصف: | Breast cancer patient-derived xenograft (BC-PDX) models represent a continuous and reproducible source of circulating tumor cells (CTCs) for studying their role in tumor biology and metastasis. We have previously shown the utility of BC-PDX models in the study of CTCs by immunohistochemistry (IHC) on serial paraffin sections and manual microscopic identification of cytokeratin-positive cells, a method that is both low-throughput and labor-intensive. We therefore aimed to identify and characterize CTCs from small volume mouse blood samples and examined its practical workflow in a study of BC-PDX mice treated with chemotherapy using an automated imaging platform, the AccuCyte®-CyteFinder® system. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000461331300001; volume:19; issue:1; firstpage:220; journal:BMC CANCER; http://hdl.handle.net/11588/771300; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85062962016; http://www.biomedcentral.com/bmccancer/ |
| DOI: | 10.1186/s12885-019-5382-1 |
| الاتاحة: | http://hdl.handle.net/11588/771300 https://doi.org/10.1186/s12885-019-5382-1 http://www.biomedcentral.com/bmccancer/ |
| رقم الانضمام: | edsbas.1D575B04 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12885-019-5382-1 |
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