Academic Journal
The Odorant Receptor Recognizing Camphor in a Camphor Tree Specialist Orthaga achatina (Lepidoptera: Pyralidae)
| العنوان: | The Odorant Receptor Recognizing Camphor in a Camphor Tree Specialist Orthaga achatina (Lepidoptera: Pyralidae) |
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| المؤلفون: | Yu Ma, Ting-Ting Yang, Shuang Ni, Ji-Xiang Wang, Yu He, Yu-Xiao Si, Jin Zhang, Shuang-Lin Dong, Qi Yan |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Genetics, Pharmacology, Evolutionary Biology, Ecology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, potential insect repellents, orco significantly responded, analyzing antenna transcriptomes, molecular docking analysis, cinnamomum camphora <, camphor tree specialist, orthaga achatina <, camphora <, camphor tree, molecular target, molecular basis, xenopus <, achatina <, totally unknown, stored products, specifically feed, ser209 residue, oocyte system, olfactory mechanisms, long history, genes obtained, female adults, effective repellent, directed mutagenesis |
| الوصف: | Camphor has been used as an effective repellent and pesticide to stored products for a long history, but Orthaga achatina (Lepidoptera: Pyralidae) has evolved to specifically feed on the camphor tree Cinnamomum camphora . However, the behavioral response of O. achatina to camphor and the molecular basis of camphor perception are totally unknown. Here, we demonstrated that both male and female adults were behaviorally attracted to camphor, suggesting the adaptation of O. achatina to and utilization of camphor as a signal of C. camphora . Second, in 40 O. achatina OR genes obtained by analyzing antenna transcriptomes, only OachOR16/Orco significantly responded to camphor in the Xenopus oocyte system. Finally, by molecular docking analysis and site-directed mutagenesis, the Ser209 residue is confirmed to be essential for binding of the oachOR16 with camphor. This study not only reveals the camphor-based host plant choice and olfactory mechanisms of O. achatina but also provides a molecular target for screening more potential insect repellents. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/The_Odorant_Receptor_Recognizing_Camphor_in_a_Camphor_Tree_Specialist_i_Orthaga_achatina_i_Lepidoptera_Pyralidae_/25058784 |
| DOI: | 10.1021/acs.jafc.3c08877.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.jafc.3c08877.s001 https://figshare.com/articles/journal_contribution/The_Odorant_Receptor_Recognizing_Camphor_in_a_Camphor_Tree_Specialist_i_Orthaga_achatina_i_Lepidoptera_Pyralidae_/25058784 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.1D52714B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jafc.3c08877.s001 |
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