Academic Journal
Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development
| العنوان: | Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development |
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| المؤلفون: | HERMANS, Florian, HEMERYCK, Lara, Bueds, Celine, Torres Pereiro, Marc, HASEVOETS, Steffie, Kobayashi, Hiroto, Lambrechts, Diether, LAMBRICHTS, Ivo, BRONCKAERS, Annelies, Vankelecom, Hugo |
| المساهمون: | HERMANS, Florian, HEMERYCK, Lara, Bueds, Celine, Torres Pereiro, Marc, HASEVOETS, Steffie, Kobayashi, Hiroto, Lambrechts, Diether, LAMBRICHTS, Ivo, BRONCKAERS, Annelies, Vankelecom, Hugo |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2023 |
| المجموعة: | Document Server@UHasselt (Universiteit Hasselt) |
| مصطلحات موضوعية: | ameloblasts, assembloids, dental epithelium, incisors, molars, organoids, single-cell RNA-sequencing, stem cells, tooth development, Animals, Mice, Humans, Molar, Cell Differentiation, Biology, Incisor |
| الوصف: | Organoid models provide powerful tools to study tissue biology and development in a dish. Presently, organoids have not yet been developed from mouse tooth. Here, we established tooth organoids (TOs) from early-postnatal mouse molar and incisor, which are long-term expandable, express dental epithelium stem cell (DESC) markers, and recapitulate key properties of the dental epithelium in a tooth-type-specific manner. TOs display in vitro differentiation capacity toward ameloblast-resembling cells, even more pronounced in assembloids in which dental mesenchymal (pulp) stem cells are combined with the organoid DESCs. Single-cell transcriptomics supports this developmental potential and reveals co-differentiation into junctional epithelium- and odontoblast-/cementoblast-like cells in the assembloids. Finally, TOs survive and show ameloblast-resembling differentiation also in vivo. The developed organoid models provide new tools to study mouse tooth-type-specific biology and development and gain deeper molecular and functional insights that may eventually help to achieve future human biological tooth repair and replacement. ; We are grateful to Evelyne Van Kerckhove (UHasselt), Marc Jans (UHasselt), Jeanine Santermans (UHasselt), and Veerle Vanslembrouck (KU Leuven) for valuable technical help. We thank Dr. Diether Lambrechts’ group (VIB,KULeuven) for technical assistance in 103 Genomics. Computational resources for transcriptome analyses were provided by the ‘‘Vlaams Supercomputer Centrum’’ (VSC), managed by the Fund for Scientific Research (FWO), Flanders (Belgium).We are also grateful to the ImagingCore (VIB,KULeuven) and the CIC (KU Leuven) for use ofmicroscopes and the Center for Brain & Disease Research (CBD) Histology unit (VIB, KU Leuven) for use of histology equipment. We acknowledge the use of the TEMplatforms at VIB-KU Leuven, UHasselt, and Tohoku University. The authors also thank Dr. Adrian Ranga (KU Leuven), Dr. Ronald Driesen(UHasselt), and other non-coauthor members of the Laboratory of Tissue ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2213-6711 |
| Relation: | Stem Cell Reports, 18 (5) , p. 1166 -1181; http://hdl.handle.net/1942/40106; 1181; 1166; 18; 001017408400001; https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(23)00099-1 |
| DOI: | 10.1016/j.stemcr.2023.03.011 |
| الاتاحة: | http://hdl.handle.net/1942/40106 https://doi.org/10.1016/j.stemcr.2023.03.011 https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(23)00099-1 |
| رقم الانضمام: | edsbas.1CD5A30B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2023.03.011 |