Academic Journal
Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China
| العنوان: | Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China |
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| المؤلفون: | Zhao Jia-Qi, Fan Ying-Ying, Lei Yun-Duan, Liu Ding, Wang Jun-Wei, Yang Xin, Song Jun-Ke, Zhao Guang-Hui |
| المصدر: | Parasite, Vol 31, p 60 (2024) |
| بيانات النشر: | EDP Sciences |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | cryptosporidium spp, giardia duodenalis, enterocytozoon bieneusi, escherichia coli, clostridium perfringens, salmonella spp, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Diarrhea caused by zoonotic pathogens is one of the most common diseases in dairy calves, threatening the health of young animals. Humans are also at risk, in particular children. To explore the pathogens causing diarrhea in dairy calves, the present study applied PCR-based sequencing tools to investigate the occurrence and molecular characteristics of three parasites (Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi) and three bacterial pathogens (Escherichia coli, Clostridium perfringens, and Salmonella spp.) in 343 fecal samples of diarrheic dairy calves from five farms in Lingwu County, Ningxia Hui Autonomous Region, China. The total positive rate of these pathogens in diarrheic dairy calves was 91.0% (312/343; 95% CI, 87.9–94.0), with C. perfringens (61.5%, 211/343; 95% CI, 56.3–66.7) being the dominant one. Co-infection with two to five pathogens was found in 67.3% (231/343; 95% CI, 62.4–72.3) of investigated samples. There were significant differences (p < 0.05) in the positive rates of Cryptosporidium spp. and diarrheagenic E. coli among farms, age groups, and seasons. Two Cryptosporidium species (C. parvum and C. bovis) and five gp60 subtypes of C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1, and a novel IIdA13G1) were identified. Two assemblages (assemblage E and zoonotic assemblage A) of G. duodenalis and six ITS genotypes of E. bieneusi (J, Henan-IV, EbpC, I, EbpA, and ESH-01) were observed. Four virulence genes (eaeA, stx1, stx2, and st) of diarrheagenic E. coli and one toxin type (type A) of C. perfringens were detected. Our study enriches our knowledge on the characteristics and zoonotic potential of diarrhea-related pathogens in dairy calves. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240098/parasite240098.html; https://doaj.org/toc/1776-1042; https://doaj.org/article/c569170427f249f3bc2aeff40f163a0f |
| DOI: | 10.1051/parasite/2024059 |
| الاتاحة: | https://doi.org/10.1051/parasite/2024059 https://doaj.org/article/c569170427f249f3bc2aeff40f163a0f |
| رقم الانضمام: | edsbas.1CC669A5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1051/parasite/2024059 |
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