التفاصيل البيبلوغرافية
| العنوان: |
Tg6F attenuates gut inflammation in humanized mice with treated HIV infection. |
| المؤلفون: |
Maria Daskou (11919515), William Mu (11013293), Madhav Sharma (11407794), Hariclea Vasilopoulos (11919518), Rachel Heymans (11919521), Eleni Ritou (3726805), Valerie Rezek (326605), Philip Hamid (10515029), Athanasios Kossyvakis (731141), Shubhendu Sen Roy (4407985), Victor Grijalva (353210), Arnab Chattopadhyay (2517265), Scott G. Kitchen (10515038), Alan M. Fogelman (11919524), Srinivasa T. Reddy (9026855), Theodoros Kelesidis (698171) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Biotechnology, Immunology, Cancer, Science Policy, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, use preclinical models, preclinical data suggest, novel therapeutic strategy, novel therapeutic strategies, mimetic peptides provide, consistently reduced plasma, adding oxidized lipoproteins, mimetic peptides 6f, mimetic peptide 6f, inflammatory mediators involved, gut tissue cytokines, ex vivo <, div >< p, 1 infected mice, mimetics reduce systemic, attenuate increased systemic, 4f attenuate systemic, chronic treated hiv, adam17 sheddase activity, potent art compared, vivo < |
| الوصف: |
TKO C57 (n = 37) humanized (BLT) mice were constructed, infected and treated with antiretroviral therapy (ART) and control transgenic tomato concentrate and/or oral ApoA-I mimetic peptide 6F in the form of transgenic tomato (Tg6F). The small intestine from each mouse was collected and protein lysates were prepared from BLT mice after 16 weeks of HIV infection in the HIV + ART + group. Human and murine cytokines [interleukin (IL)-1β, IL-6, IL-8, IL-10, tumor necrosis factor alpha (TNF-α)] and chemokines [C-C Motif Chemokine Ligand 2 (CCL2), CCL3, CCL5, C-X3-C Motif Chemokine Ligand 1 (CX3CL1), C-X-C Motif Chemokine Ligand 10 (CXCL10)] were determined in gut tissue lysates by Luminex immunoassays after 16 weeks of HIV infection in the HIV + ART + group. The mean value of each measurement in HIV + ART + (red color)(n = 14) and HIV + ART + /Tg6F + (blue color)(n = 15) mice BLT mice was expressed as fold to the mean value of each measurement in uninfected (n = 8) BLT mice (within the same cohort). A. Fold changes of human cytokines in gut tissue lysates of HIV + ART + and HIV + ART + /Tg6F + TKO mice compared to uninfected BLT mice. B. Fold changes of murine cytokines in gut tissue lysates of HIV + ART + and HIV + ART + /Tg6F + TKO mice compared to uninfected BLT mice. C. Fold changes of human chemokines in gut tissue lysates of HIV + ART + and HIV + ART + /Tg6F + TKO mice compared to uninfected BLT mice. D. Fold changes of murine chemokines in gut tissue lysates of HIV + ART + and HIV + ART + /Tg6F + TKO mice compared to uninfected BLT mice. Data represent box and whiskers with minimum, median and maximum values ( n = 8–15 mice per group). Datapoints represent mean of at least 2 experimental replicates per mouse. The Kruskal Wallis was used to compared >2 groups and the Mann-Whitney test was used to compare 2 groups (* p < 0.05, ** p < 0.01, *** p < 0.001). The asterisks in blue demonstrate the comparison relative to the uninfected group. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Tg6F_attenuates_gut_inflammation_in_humanized_mice_with_treated_HIV_infection_/18050483 |
| DOI: |
10.1371/journal.ppat.1010160.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1010160.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.1C6303F |
| قاعدة البيانات: |
BASE |