Academic Journal
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens
| العنوان: | KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
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| المؤلفون: | Caiaffa, Karina Sampaio, Massunari, Loiane, Danelon, Marcelle, Abuna, Gabriel Flores, Bedran, Telma Blanca Lombardo, Santos-Filho, Norival Alves, Spolidorio, Denise Madalena Palomari, Vizoto, Natalia Leal, Cilli, Eduardo Maffud, Duque, Cristiane |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2017 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Biofilms, cationic antimicrobial peptides, cell culture, microbial sensitivity tests |
| الوصف: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 807-818 |
| اللغة: | English |
| تدمد: | 1029-2454 0892-7014 |
| Relation: | Biofouling; 0,835; http://dx.doi.org/10.1080/08927014.2017.1370087; Biofouling, v. 33, n. 10, p. 807-818, 2017.; http://hdl.handle.net/11449/175339; 2-s2.0-85031398430; 2-s2.0-85031398430.pdf; 5651874509493617; orcid:0000-0002-2575-279X |
| DOI: | 10.1080/08927014.2017.1370087 |
| الاتاحة: | http://hdl.handle.net/11449/175339 https://doi.org/10.1080/08927014.2017.1370087 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.1C5A6949 |
| قاعدة البيانات: | BASE |
| تدمد: | 10292454 08927014 |
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| DOI: | 10.1080/08927014.2017.1370087 |