Academic Journal
Secreted Proteoglycans Directly Mediate Human Embryonic Stem Cell-Basic Fibroblast Growth Factor 2 Interactions Critical for Proliferation
| العنوان: | Secreted Proteoglycans Directly Mediate Human Embryonic Stem Cell-Basic Fibroblast Growth Factor 2 Interactions Critical for Proliferation |
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| المؤلفون: | Levenstein, Mark E., Berggren, W. Travis, Lee, Ji Eun, Conard, Kevin R., Llanas, Rachel A., Wagner, Ryan J., Smith, Lloyd M., Thomson, James A. |
| المصدر: | Stem Cells ; volume 26, issue 12, page 3099-3107 ; ISSN 1066-5099 1549-4918 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2008 |
| الوصف: | Human embryonic stem (ES) cells can be maintained in an undifferentiated state if the culture medium is first conditioned on a layer of mouse embryonic fibroblast (MEF) feeder cells. Here we show that human ES cell proliferation is coordinated by MEF-secreted heparan sulfate proteoglycans (HSPG) in conditioned medium (CM). These HSPG and other heparinoids can stabilize basic fibroblast growth factor (FGF2) in unconditioned medium at levels comparable to those observed in CM. They also directly mediate binding of FGF2 to the human ES cell surface, and their removal from CM impairs proliferation. Finally, we have developed a purification scheme for MEF-secreted HSPG in CM. Using column chromatography, immunoblotting, and mass spectrometry-based proteomic analysis, we have identified multiple HSPG species in CM. The results demonstrate that HSPG are key signaling cofactors in CM-based human ES cell culture. Disclosure of potential conflicts of interest is found at the end of this article. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1634/stemcells.2007-1056 |
| الاتاحة: | http://dx.doi.org/10.1634/stemcells.2007-1056 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1634%2Fstemcells.2007-1056 https://onlinelibrary.wiley.com/doi/pdf/10.1634/stemcells.2007-1056 https://onlinelibrary.wiley.com/doi/full-xml/10.1634/stemcells.2007-1056 https://academic.oup.com/stmcls/article-pdf/26/12/3099/41943617/stmcls_26_12_3099.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.1C5A17EE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1634/stemcells.2007-1056 |
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