Academic Journal
Porcine Hemagglutinating Encephalomyelitis Virus Infection In Vivo and Ex Vivo.
| العنوان: | Porcine Hemagglutinating Encephalomyelitis Virus Infection In Vivo and Ex Vivo. |
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| المؤلفون: | Mora-Díaz, Juan Carlos, Piñeyro, Pablo E, Rauh, Rolf, Nelson, William, Sankoh, Zianab, Gregg, Edward, Carrillo-Ávila, José Antonio, Shen, Huigang, Nelli, Rahul K, Zimmerman, Jeffrey J, Giménez-Lirola, Luis G |
| سنة النشر: | 2021 |
| المجموعة: | Sistema Sanitario Público de Andalucía (SSPA): Repositorio |
| مصطلحات موضوعية: | CDCD (cesarean derived, air-liquid interface respiratory epithelial cells, betacoronavirus, colostrum deprived), coronavirus, infection, neonatal pigs, porcine hemagglutinating encephalomyelitis virus, upper respiratory tract, Animals, Betacoronavirus 1, Cell Line, Coronavirus Infections, Interferon-alpha, Interleukin-8, Organ Specificity, Reverse Transcriptase Polymerase Chain Reaction, Swine, Swine Diseases, T-Lymphocytes |
| الوصف: | Porcine hemagglutinating encephalomyelitis virus (PHEV) is a betacoronavirus that causes vomiting and wasting disease and/or encephalomyelitis in suckling pigs. This study characterized PHEV infection, pathogenesis, and immune response in cesarean-derived, colostrum-deprived (CDCD) neonatal pigs. Infected animals developed mild respiratory, enteric, and neurological clinical signs between 2 to 13 days postoronasal inoculation (dpi). PHEV did not produce viremia, but virus shedding was detected in nasal secretions (1 to 10 dpi) and feces (2 to 7 dpi) by reverse transcriptase quantitative PCR (RT-qPCR). Viral RNA was detected in all tissues except liver, but the detection rate and RT-qPCR threshold cycle (CT ) values decreased over time. The highest concentration of virus was detected in inoculated piglets necropsied at 5 dpi in turbinate and trachea, followed by tonsils, lungs, tracheobronchial lymph nodes, and stomach. The most representative microscopic lesions were gastritis lymphoplasmacytic, moderate, multifocal, with perivasculitis, and neuritis with ganglia degeneration. A moderate inflammatory response, characterized by increased levels of interferon alpha (IFN-α) in plasma (5 dpi) and infiltration of T lymphocytes and macrophages were also observed. Increased plasma levels of interleukin-8 (IL-8) were detected at 10 and 15 dpi, coinciding with the progressive resolution of the infection. Moreover, a robust antibody response was detected by 10 dpi. An ex vivo air-liquid CDCD-derived porcine respiratory cells culture (ALI-PRECs) system showed virus replication in ALI-PRECs and cytopathic changes and disruption of ciliated columnar epithelia, thereby confirming the tracheal epithelia as a primary site of infection for PHEV.IMPORTANCE Among the ∼46 virus species in the family Coronaviridae, many of which are important pathogens of humans and 6 of which are commonly found in pigs, porcine hemagglutinating encephalomyelitis remains one of the least researched. The present study provided a comprehensive ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1098-5514 |
| Relation: | http://hdl.handle.net/10668/17397; PMC8316118; https://doi.org/10.1128/jvi.02335-20; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316118/pdf |
| DOI: | 10.1128/JVI.02335-20 |
| الاتاحة: | http://hdl.handle.net/10668/17397 https://doi.org/10.1128/JVI.02335-20 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316118/pdf |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; open access |
| رقم الانضمام: | edsbas.1C587638 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10985514 |
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| DOI: | 10.1128/JVI.02335-20 |