Dissertation/ Thesis
Study of the stress response capacity at the cellular level of the symbiotic Cnidaria Anemonia viridis ; Étude de la capacité de réponse au stress à l'échelle cellulaire du Cnidaire symbiotique Anemonia viridis
| العنوان: | Study of the stress response capacity at the cellular level of the symbiotic Cnidaria Anemonia viridis ; Étude de la capacité de réponse au stress à l'échelle cellulaire du Cnidaire symbiotique Anemonia viridis |
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| المؤلفون: | Cotinat, Pauline |
| المساهمون: | Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Université Côte d'Azur, Paola Furla, Stéphanie Barnay-Verdier |
| المصدر: | https://theses.hal.science/tel-04099820 ; Biologie cellulaire. Université Côte d'Azur, 2023. Français. ⟨NNT : 2023COAZ6003⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | HAL Université Côte d'Azur |
| مصطلحات موضوعية: | Cell culture, Oxidative stress, Symbiosis, Sunscreens, Senescence, Hydrogen peroxide, Culture cellulaire, Stress oxydant, Symbiose, Crèmes solaires, Sénescence, Peroxyde d'hydrogène, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology |
| الوصف: | The objective of this thesis was to characterize the response of the cells of a symbiotic Cnidaria, the sea anemone, Anemonia viridis, to extrinsic stresses related to the anthropic pressure of coastal areas, and intrinsic stresses related to the constraints of living in symbiosis. Indeed, the evolutionary success of symbiosis in Cnidaria is based on the photosynthetic activity of the symbiont (a microalga of the Symbiodiniaceae family), which also presents constraints, such as a daily exposure of the animal tissue to pro-oxidant conditions due to the production of O2 during photosynthesis.We were first interested in the response capacity of cultured A. viridis cells to a stress related to pollution by solar products. An innovative in vitro marine ecotoxicology assay based on the monitoring of cell parameters, such as viability and growth, allowed us to evaluate the ecotoxicity of several types of sunscreen products, namely: 1) raw materials, namely the UV filters Avobenzone and Benzophenone-3, with EC50 values in agreement with the literature; 2) formulation blocks and 3) finished products with a wide range of responses, validating the sensitivity of this approach to assess the toxicity of sunscreen products on the coastal marine ecosystem. Thanks to this test and by combining a preventive strategy of eco-design within the company SO.F.I.A Cosmétiques, we participated in the formulation of a range of more eco-responsible sunscreen products.Subsequently, we analyzed the ability of A. viridis to respond to pro-oxidant conditions by in vivo and in vitro approaches. Whole specimens and cell cultures were subjected to 200 and 500μM H2O2 for 7 days, and then we measured global health parameters (symbiotic status, cell viability and growth) and stress biomarkers (antioxidant capacity, protein damage). At the whole organism level, both H2O2 concentrations did not affect survival and no bleaching was observed. Only the symbiont compartment exhibited oxidative protein damage after a 7-day exposure to 500μM H2O2, despite ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| Relation: | NNT: 2023COAZ6003; tel-04099820; https://theses.hal.science/tel-04099820; https://theses.hal.science/tel-04099820/document; https://theses.hal.science/tel-04099820/file/2023COAZ6003.pdf |
| الاتاحة: | https://theses.hal.science/tel-04099820 https://theses.hal.science/tel-04099820/document https://theses.hal.science/tel-04099820/file/2023COAZ6003.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.1BCEEF7A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |