Academic Journal
Enzyme‐Responsive LipoCEST Agents: Assessment of MMP‐2 Activity by Measuring the Intra‐liposomal Water 1 H NMR Shift
| العنوان: | Enzyme‐Responsive LipoCEST Agents: Assessment of MMP‐2 Activity by Measuring the Intra‐liposomal Water 1 H NMR Shift |
|---|---|
| المؤلفون: | Ferrauto, Giuseppe, Di Gregorio, Enza, Ruzza, Marta, Catanzaro, Valeria, Padovan, Sergio, Aime, Silvio |
| المساهمون: | Horizon 2020 Framework Programme, Fondazione Italiana per la Ricerca sul Cancro |
| المصدر: | Angewandte Chemie International Edition ; volume 56, issue 40, page 12170-12173 ; ISSN 1433-7851 1521-3773 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Mobile proton‐containing solutes can be detected by MRI by the chemical exchange saturation transfer (CEST) method. CEST sensitivity is dramatically enhanced by using, as exchanging protons, the water molecules confined inside liposomes, shifted by a paramagnetic shift reagent. The chemical shift of the intraliposomal water resonance (δ IL ) is affected by the overall shape of the supramolecular system. δ IL of a spherical LipoCEST acts as a sensitive reporter of the distribution of streptavidin proteins anchored at the liposome surface by biotinylated phospholipids. This finding prompted the design of a MMP‐2 responsive LipoCEST agent as the streptavidin moieties can be released from the liposome surfaces when a properly tailored enzyme‐cleavable peptide is inserted on the phospholipids before the terminal biotin residues. δ IL reports on the overall changes in the supramolecular architecture associated to the cleavage carried out by MMP‐2. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/anie.201706271 |
| الاتاحة: | http://dx.doi.org/10.1002/anie.201706271 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201706271 https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201706271 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.1B3DE1E6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/anie.201706271 |
|---|