Academic Journal
Cerium-based Histochemical Demonstration of Oxidative Stress in Taurocholate-induced Acute Pancreatitis in Rats: A Confocal Laser Scanning Microscopic Study
| العنوان: | Cerium-based Histochemical Demonstration of Oxidative Stress in Taurocholate-induced Acute Pancreatitis in Rats: A Confocal Laser Scanning Microscopic Study |
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| المؤلفون: | Telek, Géza, Scoazec, Jean-Yves, Chariot, Jacques, Ducroc, Robert, Feldmann, Gerard, Rozé, Claude |
| المصدر: | Journal of Histochemistry & Cytochemistry ; volume 47, issue 9, page 1201-1212 ; ISSN 0022-1554 1551-5044 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 1999 |
| الوصف: | Direct in vivo histological detection of oxygen-derived free radicals (OFRs) in inflammatory conditions is not fully resolved. We report an application of cerium histochemistry (in which capture of OFRs by Ce atoms results in laser-reflectant cerium-perhydroxide precipitates) combined with reflectance confocal laser scanning microscopy (CLSM) to demonstrate the evolution of oxidative stress in taurocholate-induced acute pancreatitis (AP) in rats. Animals were perfused with CeCl 3 in vivo and cryostat sections of pancreata were studied by CLSM. Vascular endothelium was immunolabeled for PECAM-1. OFR production by isolated polymorphonuclear leukocytes (PMNs) incubated in vitro with CeCl 3 was quantified by image analysis. In the pancreas, strong OFR-derived cerium reflectance signals were seen in acinar cells at 1-2 hr, capillaries and small venules were frequently engorged by cerium precipitates, and adherent PMNs presented weak intracellular reflectance signals. At 8-24 hr, acinar cell OFR production decreased, whereas adherent/transmigrated PMNs displayed abundant intra- and pericellular reflectance. PECAM-1 expression was unchanged. PMNs from ascites or blood showed significant ( p<0.01) time-dependent OFR production, plateauing from 2 hr. The modified cerium capture/CLSM method allows the co-demonstration of in vivo oxidative stress and cellular structures labeled with fluorescent markers. In vivo oxidative stress was shown histologically for the first time in experimental AP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/002215549904700912 |
| الاتاحة: | http://dx.doi.org/10.1177/002215549904700912 http://journals.sagepub.com/doi/pdf/10.1177/002215549904700912 http://journals.sagepub.com/doi/full-xml/10.1177/002215549904700912 |
| Rights: | http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.1B37441B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/002215549904700912 |
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