Academic Journal
Innovative in vitro method to study ventilator induced lung injury.
| العنوان: | Innovative in vitro method to study ventilator induced lung injury. |
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| المؤلفون: | Joelsson, Jon P, Myszor, Iwona T, Arason, Ari J, Ingthorsson, Saevar, Cherek, Paulina, Windels, Gabriel S, Leosson, Kristjan, Gudmundsson, Gudmundur H, Gudjonsson, Thorarinn, Karason, Sigurbergur |
| المساهمون: | 1Stem Cell Research Unit, BioMedical Centre, School of Health Sciences, University of Iceland, Reykjavík, Iceland. 2Department of Laboratory Hematology, Landspitali-University Hospital, Reykjavík, Iceland. 3Life and Environmental Sciences, BioMedical Centre, University of Iceland, Reykjavík, Iceland. 4Innovation Centre Iceland, Reykjavík, Iceland. 5Intensive Care Unit, Landspitali-University Hospital, Reykjavík, Iceland. |
| المصدر: | ALTEX ; 36 ; 4 ; 634 ; 642 ; Germany |
| بيانات النشر: | Spektrum Akademischer Verlag |
| سنة النشر: | 2020 |
| المجموعة: | Hirsla - Landspítali University Hospital research archive |
| مصطلحات موضوعية: | VILI, ARDS, lung disease, bronchial epithelium, Öndunarvélar, Lungnasjúkdómar, Respiration, Artificial, Lung Injury |
| الوصف: | To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download ; Mechanical ventilation (MV) is a life-saving therapy for critically ill patients, alleviating the work of breathing and supporting adequate gas exchange. However, MV can cause ventilator induced lung injury (VILI) by baro/volu- and atelectrauma, even lead to acute respiratory distress syndrome (ARDS), and substantially augment mortality. There is a need for specific biomarkers and novel research platforms for VILI/ARDS research to study these detrimental disorders and seek ways to avoid or prevent them. Previous in vitro studies on bronchial epithelium, cultured in air-liquid interface (ALI) conditions, have generally utilized static or constant pressure. We have developed a Cyclical Pressure ALI Device (CPAD) that enables cyclical stress on ALI cultured human bronchial cells, with the aim of mimicking the effects of MV. Using CPAD we were able to analyze differentially expressed VILI/ARDS and innate immunity associated genes along with increased expression of associated proteins. CPAD provides an easy and accessible way to analyze functional and phenotypic changes that occur during VILI and may provide a platform for future drug testing. ; Technology development fund - Icelandic research council University of Iceland Land-spitali, University Hospital, Science fund |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1868-596X |
| Relation: | https://www.altex.org/index.php/altex/article/view/1245; Joelsson JP, Myszor IT, Arason AJ, et al. Innovative in vitro method to study ventilator induced lung injury. ALTEX. 2019;36(4):634–642. doi:10.14573/altex.1901182; http://hdl.handle.net/2336/621313; ALTEX |
| DOI: | 10.14573/altex.1901182 |
| الاتاحة: | http://hdl.handle.net/2336/621313 https://doi.org/10.14573/altex.1901182 |
| Rights: | Open Access - Opinn aðgangur |
| رقم الانضمام: | edsbas.1B070099 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1868596X |
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| DOI: | 10.14573/altex.1901182 |