Academic Journal
Transcriptome dynamics of CD4+ T cells during malaria maps gradual transit from effector to memory
| العنوان: | Transcriptome dynamics of CD4+ T cells during malaria maps gradual transit from effector to memory |
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| المؤلفون: | Soon, Megan SF, Lee, Hyun Jae, Engel, Jessica A, Straube, Jasmin, Thomas, Bryce S, Pernold, Clara PS, Clarke, Lachlan S, Laohamonthonkul, Pawat, Haldar, Rohit N, Williams, Cameron G, Lansink, Lianne IM, Moreira, Marcela L, Bramhall, Michael, Engwerda, Christian R |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2020 |
| المجموعة: | Griffith University: Griffith Research Online |
| مصطلحات موضوعية: | Immunology |
| الوصف: | The dynamics of CD4+ T cell memory development remain to be examined at genome scale. In malaria-endemic regions, antimalarial chemoprevention protects long after its cessation and associates with effects on CD4+ T cells. We applied single-cell RNA sequencing and computational modelling to track memory development during Plasmodium infection and treatment. In the absence of central memory precursors, two trajectories developed as T helper 1 (TH1) and follicular helper T (TFH) transcriptomes contracted and partially coalesced over three weeks. Progeny of single clones populated TH1 and TFH trajectories, and fate-mapping suggested that there was minimal lineage plasticity. Relationships between TFH and central memory were revealed, with antimalarials modulating these responses and boosting TH1 recall. Finally, single-cell epigenomics confirmed that heterogeneity among effectors was partially reset in memory. Thus, the effector-to-memory transition in CD4+ T cells is gradual during malaria and is modulated by antiparasitic drugs. Graphical user interfaces are presented for examining gene-expression dynamics and gene-gene correlations ( http://haquelab.mdhs.unimelb.edu.au/cd4_memory/ ). ; No Full Text |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1529-2908 |
| Relation: | Nature Immunology; Soon, MSF; Lee, HJ; Engel, JA; Straube, J; Thomas, BS; Pernold, CPS; Clarke, LS; Laohamonthonkul, P; Haldar, RN; Williams, CG; Lansink, LIM; Moreira, ML; Bramhall, M; Engwerda, CR; et al., Transcriptome dynamics of CD4+ T cells during malaria maps gradual transit from effector to memory, Nature Immunology, 2020; http://hdl.handle.net/10072/398565 |
| DOI: | 10.1038/s41590-020-0800-8 |
| الاتاحة: | http://hdl.handle.net/10072/398565 https://doi.org/10.1038/s41590-020-0800-8 |
| Rights: | open access |
| رقم الانضمام: | edsbas.1AED42FE |
| قاعدة البيانات: | BASE |
| تدمد: | 15292908 |
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| DOI: | 10.1038/s41590-020-0800-8 |