التفاصيل البيبلوغرافية
| العنوان: |
Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control |
| المؤلفون: |
Themistoklis Zisis (1522426), Jan Schwarz (5746046), Miriam Balles (11160818), Maibritt Kretschmer (11160821), Maria Nemethova (222097), Remy Chait (235728), Robert Hauschild (2441194), Janina Lange (323354), Calin Guet (11160824), Michael Sixt (92958), Stefan Zahler (288655) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Physiology, Biotechnology, Immunology, Developmental Biology, Cancer, Inorganic Chemistry, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, light-dose-dependent patterning step, switchable patterning step, Spatiotemporal Control Attachment, spatiotemporal control, method, high-throughput covalent photoimmob., sequential photopatterning approach, cell culture surfaces |
| الوصف: |
Attachment of adhesive molecules on cell culture surfaces to restrict cell adhesion to defined areas and shapes has been vital for the progress of in vitro research. In currently existing patterning methods, a combination of pattern properties such as stability, precision, specificity, high-throughput outcome, and spatiotemporal control is highly desirable but challenging to achieve. Here, we introduce a versatile and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent patterning step and a subsequent functionalization of the pattern via click chemistry. This two-step process is feasible on arbitrary surfaces and allows for generation of sustainable patterns and gradients. The method is validated in different biological systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining the growth and migration of cells to the designated areas. We then implement a sequential photopatterning approach by adding a second switchable patterning step, allowing for spatiotemporal control over two distinct surface patterns. As a proof of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis. Our results show that the spatiotemporal control provided by our “sequential photopatterning” system is essential for mimicking dynamic biological processes and that our innovative approach has great potential for further applications in cell science. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Sequential_and_Switchable_Patterning_for_Studying_Cellular_Processes_under_Spatiotemporal_Control/15025199 |
| DOI: |
10.1021/acsami.1c09850.s001 |
| الاتاحة: |
https://doi.org/10.1021/acsami.1c09850.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.1AEA6769 |
| قاعدة البيانات: |
BASE |